6n7w
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6n7w is ON HOLD Authors: Gao, Y., Fox, T., Val, N., Yang, W. Description: D5AE7A mutant gp5 DNA polymerase complexed with trx, fork DNA, and incomi...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)== | |
| + | <SX load='6n7w' size='340' side='right' viewer='molstar' caption='[[6n7w]], [[Resolution|resolution]] 4.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6n7w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N7W FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n7w OCA], [https://pdbe.org/6n7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n7w RCSB], [https://www.ebi.ac.uk/pdbsum/6n7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n7w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DPOL_BPT7 DPOL_BPT7] Replicates viral genomic DNA. Non-processive DNA polymerase that achieves processivity by binding to host thioredoxin (TrxA). This interaction increases the rate of dNTP incorporation to yield a processivity of approximately 800 nucleotides (nt) per binding event. Interacts with DNA helicase gp4 to coordinate nucleotide polymerization with unwinding of the DNA. The leading strand is synthesized continuously while synthesis of the lagging strand requires the synthesis of oligoribonucleotides by the primase domain of gp4.<ref>PMID:9218486</ref> <ref>PMID:21606333</ref> | ||
| - | + | ==See Also== | |
| - | + | *[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | |
| - | + | *[[Thioredoxin 3D structures|Thioredoxin 3D structures]] | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Fox | + | </SX> |
| - | [[Category: Yang | + | [[Category: Escherichia coli]] |
| + | [[Category: Escherichia phage T7]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fox T]] | ||
| + | [[Category: Gao Y]] | ||
| + | [[Category: Val N]] | ||
| + | [[Category: Yang W]] | ||
Current revision
Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes)
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