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6o3h

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==Icosahedral reconstruction of the thermophilic bacteriophage P74-26 capsid==
==Icosahedral reconstruction of the thermophilic bacteriophage P74-26 capsid==
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<StructureSection load='6o3h' size='340' side='right'caption='[[6o3h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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<SX load='6o3h' size='340' side='right' viewer='molstar' caption='[[6o3h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6o3h]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_virus_p74-26 Thermus virus p74-26]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O3H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O3H FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6o3h]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_virus_P74-26 Thermus virus P74-26]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O3H FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o3h OCA], [http://pdbe.org/6o3h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o3h RCSB], [http://www.ebi.ac.uk/pdbsum/6o3h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o3h ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o3h OCA], [https://pdbe.org/6o3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o3h RCSB], [https://www.ebi.ac.uk/pdbsum/6o3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o3h ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A7XXR6_BP742 A7XXR6_BP742]
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The capsids of double-stranded DNA viruses protect the viral genome from the harsh extracellular environment, while maintaining stability against the high internal pressure of packaged DNA. To elucidate how capsids maintain stability in an extreme environment, we use cryoelectron microscopy to determine the capsid structure of thermostable phage P74-26 to 2.8-A resolution. We find P74-26 capsids exhibit an overall architecture very similar to those of other tailed bacteriophages, allowing us to directly compare structures to derive the structural basis for enhanced stability. Our structure reveals lasso-like interactions that appear to function like catch bonds. This architecture allows the capsid to expand during genome packaging, yet maintain structural stability. The P74-26 capsid has T = 7 geometry despite being twice as large as mesophilic homologs. Capsid capacity is increased with a larger, flatter major capsid protein. Given these results, we predict decreased icosahedral complexity (i.e. T &lt;/= 7) leads to a more stable capsid assembly.
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Principles for enhancing virus capsid capacity and stability from a thermophilic virus capsid structure.,Stone NP, Demo G, Agnello E, Kelch BA Nat Commun. 2019 Oct 2;10(1):4471. doi: 10.1038/s41467-019-12341-z. PMID:31578335<ref>PMID:31578335</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6o3h" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thermus virus p74-26]]
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[[Category: Thermus virus P74-26]]
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[[Category: Agnello, E]]
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[[Category: Agnello E]]
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[[Category: Demo, G]]
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[[Category: Demo G]]
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[[Category: Kelch, B A]]
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[[Category: Kelch BA]]
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[[Category: Stone, N P]]
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[[Category: Stone NP]]
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[[Category: Bacteriophage]]
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[[Category: Capsid]]
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[[Category: Caudovirus]]
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[[Category: Decoration protein]]
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[[Category: Icosahedral virus]]
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[[Category: Major capsid protein]]
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[[Category: Thermophilic]]
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[[Category: Virion]]
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[[Category: Virus]]
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Current revision

Icosahedral reconstruction of the thermophilic bacteriophage P74-26 capsid

6o3h, resolution 2.80Å

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