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6pew

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==CryoEM Plasmodium falciparum glutamine synthetase==
==CryoEM Plasmodium falciparum glutamine synthetase==
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<StructureSection load='6pew' size='340' side='right'caption='[[6pew]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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<SX load='6pew' size='340' side='right' viewer='molstar' caption='[[6pew]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6pew]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum_(isolate_nf54) Plasmodium falciparum (isolate nf54)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PEW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PEW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6pew]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_NF54 Plasmodium falciparum NF54]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PEW FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pew OCA], [http://pdbe.org/6pew PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pew RCSB], [http://www.ebi.ac.uk/pdbsum/6pew PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pew ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pew OCA], [https://pdbe.org/6pew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pew RCSB], [https://www.ebi.ac.uk/pdbsum/6pew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pew ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/W7JM41_PLAFO W7JM41_PLAFO]
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X-ray crystallography often requires non-native constructs involving mutations or truncations, and is challenged by membrane proteins and large multicomponent complexes. We present here a bottom-up endogenous structural proteomics approach whereby near-atomic-resolution cryo electron microscopy (cryoEM) maps are reconstructed ab initio from unidentified protein complexes enriched directly from the endogenous cellular milieu, followed by identification and atomic modeling of the proteins. The proteins in each complex are identified using cryoID, a program we developed to identify proteins in ab initio cryoEM maps. As a proof of principle, we applied this approach to the malaria-causing parasite Plasmodium falciparum, an organism that has resisted conventional structural-biology approaches, to obtain atomic models of multiple protein complexes implicated in intraerythrocytic survival of the parasite. Our approach is broadly applicable for determining structures of undiscovered protein complexes enriched directly from endogenous sources.
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Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu.,Ho CM, Li X, Lai M, Terwilliger TC, Beck JR, Wohlschlegel J, Goldberg DE, Fitzpatrick AWP, Zhou ZH Nat Methods. 2019 Nov 25. pii: 10.1038/s41592-019-0637-y. doi:, 10.1038/s41592-019-0637-y. PMID:31768063<ref>PMID:31768063</ref>
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==See Also==
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*[[Glutamine synthetase 3D structures|Glutamine synthetase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6pew" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ho, C M]]
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[[Category: Plasmodium falciparum NF54]]
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[[Category: Lai, M]]
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[[Category: Ho CM]]
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[[Category: Zhou, Z H]]
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[[Category: Lai M]]
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[[Category: Glutamine synthetase]]
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[[Category: Zhou ZH]]
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[[Category: Ligase]]
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Current revision

CryoEM Plasmodium falciparum glutamine synthetase

6pew, resolution 3.20Å

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