This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1doa

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:51, 20 March 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1doa.gif|left|200px]]
 
-
{{Structure
+
==Structure of the rho family gtp-binding protein cdc42 in complex with the multifunctional regulator rhogdi==
-
|PDB= 1doa |SIZE=350|CAPTION= <scene name='initialview01'>1doa</scene>, resolution 2.60&Aring;
+
<StructureSection load='1doa' size='340' side='right'caption='[[1doa]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=GER:GERAN-8-YL GERAN'>GER</scene>
+
<table><tr><td colspan='2'>[[1doa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DOA FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMT:O-METHYLCYSTEINE'>CMT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GER:GERAN-8-YL+GERAN'>GER</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1doa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1doa OCA], [https://pdbe.org/1doa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1doa RCSB], [https://www.ebi.ac.uk/pdbsum/1doa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1doa ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/do/1doa_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1doa ConSurf].
 +
<div style="clear:both"></div>
-
'''STRUCTURE OF THE RHO FAMILY GTP-BINDING PROTEIN CDC42 IN COMPLEX WITH THE MULTIFUNCTIONAL REGULATOR RHOGDI'''
+
==See Also==
-
 
+
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
-
 
+
*[[Guanine nucleotide dissociation inhibitor|Guanine nucleotide dissociation inhibitor]]
-
==Overview==
+
== References ==
-
The RhoGDI proteins serve as key multifunctional regulators of Rho family GTP-binding proteins. The 2.6 A X-ray crystallographic structure of the Cdc42/RhoGDI complex reveals two important sites of interaction between GDI and Cdc42. First, the amino-terminal regulatory arm of the GDI binds to the switch I and II domains of Cdc42 leading to the inhibition of both GDP dissociation and GTP hydrolysis. Second, the geranylgeranyl moiety of Cdc42 inserts into a hydrophobic pocket within the immunoglobulin-like domain of the GDI molecule leading to membrane release. The structural data demonstrate how GDIs serve as negative regulators of small GTP-binding proteins and how the isoprenoid moiety is utilized in this critical regulatory interaction.
+
<references/>
-
 
+
__TOC__
-
==About this Structure==
+
</StructureSection>
-
1DOA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOA OCA].
+
-
 
+
-
==Reference==
+
-
Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI., Hoffman GR, Nassar N, Cerione RA, Cell. 2000 Feb 4;100(3):345-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10676816 10676816]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Cerione, R C.]]
+
[[Category: Cerione RC]]
-
[[Category: Hoffman, G R.]]
+
[[Category: Hoffman GR]]
-
[[Category: Nassar, N.]]
+
[[Category: Nassar N]]
-
[[Category: GDP]]
+
-
[[Category: GER]]
+
-
[[Category: MG]]
+
-
[[Category: cdc42]]
+
-
[[Category: gtp-binding protein]]
+
-
[[Category: rhogdi]]
+
-
[[Category: x-ray]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 11:32:22 2008''
+

Current revision

Structure of the rho family gtp-binding protein cdc42 in complex with the multifunctional regulator rhogdi

PDB ID 1doa

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1doa"
Personal tools