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1dtk

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[[Image:1dtk.gif|left|200px]]<br /><applet load="1dtk" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dtk" />
 
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'''THE NMR SOLUTION STRUCTURE OF DENDROTOXIN K FROM THE VENOM OF DENDROASPIS POLYLEPIS POLYLEPIS'''<br />
 
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==Overview==
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==THE NMR SOLUTION STRUCTURE OF DENDROTOXIN K FROM THE VENOM OF DENDROASPIS POLYLEPIS POLYLEPIS==
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The solution structure of dendrotoxin K (Toxin K), a protein consisting of one polypeptide chain with 57 residues purified from the venom of the black mamba, Dendroaspis polylepis polylepis, was determined by nuclear magnetic resonance (NMR) spectroscopy. On the basis of virtually complete sequence-specific 1H NMR assignments, including individual assignments for 38 pairs of diastereotopic substituents and side-chain amide protons, a total of 818 nuclear Overhauser effect distance constraints and 123 dihedral angle constraints were identified. Using this input, the solution structure of Toxin K was calculated with the program DIANA, and refined by restrained energy-minimization with a modified version of the program AMBER. The average root-mean-square deviation (r.m.s.d.) relative to the mean atomic co-ordinates of the 20 conformers selected to represent the solution structure is 0.31 A for all backbone atoms N, C alpha and C', and 0.90 A for all heavy-atoms of residues 2 to 56. The solution structure of Toxin K is very similar to the solution structure of the basic pancreatic trypsin inhibitor (BPTI) and the X-ray crystal structure of the alpha-dendrotoxin from Dendroaspis angusticeps (alpha-DTX), with r.m.s.d. values of 1.31 A and 0.92 A, respectively, for the backbone atoms of residues 2 to 56. Some local structural differences between Toxin K and BPTI are directly related to the fact that intermolecular interactions with two of the four internal molecules of hydration water in BPTI are replaced by intramolecular hydrogen bonds in Toxin K.
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<StructureSection load='1dtk' size='340' side='right'caption='[[1dtk]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dtk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendroaspis_polylepis_polylepis Dendroaspis polylepis polylepis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DTK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dtk OCA], [https://pdbe.org/1dtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dtk RCSB], [https://www.ebi.ac.uk/pdbsum/1dtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dtk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VKTHK_DENPO VKTHK_DENPO] Serine protease inhibitor homolog that selectively blocks voltage-gated potassium channels homooligomer Kv1.1/KCNA1 (EC(50)=0.6 nM) and Kv1.1-containing heterooligomer.<ref>PMID:10429207</ref> <ref>PMID:8612784</ref> <ref>PMID:9134213</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dtk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dtk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DTK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dendroaspis_polylepis_polylepis Dendroaspis polylepis polylepis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTK OCA].
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*[[Dendrotoxin|Dendrotoxin]]
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== References ==
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==Reference==
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<references/>
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Nuclear magnetic resonance solution structure of dendrotoxin K from the venom of Dendroaspis polylepis polylepis., Berndt KD, Guntert P, Wuthrich K, J Mol Biol. 1993 Dec 5;234(3):735-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8254670 8254670]
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__TOC__
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</StructureSection>
[[Category: Dendroaspis polylepis polylepis]]
[[Category: Dendroaspis polylepis polylepis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Berndt, K.]]
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[[Category: Berndt K]]
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[[Category: Guntert, P.]]
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[[Category: Guntert P]]
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[[Category: Wuthrich, K.]]
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[[Category: Wuthrich K]]
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[[Category: presynaptic neurotoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:16 2008''
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Current revision

THE NMR SOLUTION STRUCTURE OF DENDROTOXIN K FROM THE VENOM OF DENDROASPIS POLYLEPIS POLYLEPIS

PDB ID 1dtk

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