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1dv8

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(New page: 200px<br /> <applet load="1dv8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dv8, resolution 2.30&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1dv8.gif|left|200px]]<br />
 
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<applet load="1dv8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dv8, resolution 2.30&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE CARBOHYDRATE RECOGNITION DOMAIN OF THE H1 SUBUNIT OF THE ASIALOGLYCOPROTEIN RECEPTOR'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE CARBOHYDRATE RECOGNITION DOMAIN OF THE H1 SUBUNIT OF THE ASIALOGLYCOPROTEIN RECEPTOR==
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The human asialoglycoprotein receptor (ASGPR), also called hepatic lectin, is an integral membrane protein and is responsible for the clearance of, desialylated, galactose-terminal glycoproteins from the circulation by, receptor-mediated endocytosis. It can be subdivided into four functional, domains: the cytosolic domain, the transmembrane domain, the stalk and the, carbohydrate recognition domain (CRD). The galactose-binding domains, belong to the superfamily of C-type (calcium-dependent) lectins, in, particular to the long-form subfamily with three conserved intramolecular, disulphide bonds. It is able to bind terminal non-reducing galactose, residues and N-acetyl-galactosamine residues of desialated tri or, tetra-antennary N-linked glycans. The ASGPR is a potential liver-specific, receptor for hepatitis B virus and Marburg virus and has been used to, target exogenous molecules specifically to hepatocytes for diagnostic and, therapeutic purposes.Here, we present the X-ray crystal structure of the, carbohydrate recognition domain of the major subunit H1 at 2.3 A, resolution. While the overall fold of this and other known C-type lectin, structures are well conserved, the positions of the bound calcium ions are, not, indicating that the fold is stabilised by alternative mechanisms in, different branches of the C-type lectin family. It is the first CRD, structure where three calcium ions form an intergral part of the, structure. In addition, the structure provides direct confirmation for the, conversion of the ligand-binding site of the mannose-binding protein to an, asialoglycoprotein receptor-like specificity suggested by Drickamer and, colleagues. In agreement with the prediction that the coiled-coil domain, of the ASGPR is separated from the CRD and its N-terminal disulphide, bridge by several residues, these residues are indeed not alpha-helical, while in tetranectin they form an alpha-helical coiled-coil.
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<StructureSection load='1dv8' size='340' side='right'caption='[[1dv8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1dv8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DV8 FirstGlance]. <br>
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1DV8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DV8 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dv8 OCA], [https://pdbe.org/1dv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dv8 RCSB], [https://www.ebi.ac.uk/pdbsum/1dv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dv8 ProSAT]</span></td></tr>
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Crystal structure of the carbohydrate recognition domain of the H1 subunit of the asialoglycoprotein receptor., Meier M, Bider MD, Malashkevich VN, Spiess M, Burkhard P, J Mol Biol. 2000 Jul 21;300(4):857-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10891274 10891274]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASGR1_HUMAN ASGR1_HUMAN] Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dv/1dv8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dv8 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bider, M.D.]]
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[[Category: Bider MD]]
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[[Category: Burkhard, P.]]
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[[Category: Burkhard P]]
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[[Category: Malashkevich, V.N.]]
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[[Category: Malashkevich VN]]
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[[Category: Meier, M.]]
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[[Category: Meier M]]
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[[Category: Spiess, M.]]
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[[Category: Spiess M]]
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[[Category: CA]]
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[[Category: CL]]
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[[Category: c-type lectin crd]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:36:16 2007''
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Current revision

CRYSTAL STRUCTURE OF THE CARBOHYDRATE RECOGNITION DOMAIN OF THE H1 SUBUNIT OF THE ASIALOGLYCOPROTEIN RECEPTOR

PDB ID 1dv8

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