1dzo

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(New page: 200px<br /><applet load="1dzo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dzo, resolution 1.63&Aring;" /> '''TRUNCATED PAK PILIN ...)
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[[Image:1dzo.jpg|left|200px]]<br /><applet load="1dzo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dzo, resolution 1.63&Aring;" />
 
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'''TRUNCATED PAK PILIN FROM PSEUDOMONAS AERUGINOSA'''<br />
 
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==Overview==
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==Truncated PAK pilin from Pseudomonas aeruginosa==
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Fibers of pilin monomers (pili) form the dominant adhesin of Pseudomonas, aeruginosa, and they play an important role in infections by this, opportunistic bacterial pathogen. Blocking adhesion is therefore a target, for vaccine development. The receptor-binding site is located in a, C-terminal disulphide-bonded loop of each pilin monomer, but functional, binding sites are displayed only at the tip of the pilus. A factor, complicating vaccination is that different bacterial strains produce, distinct, and sometimes highly divergent, pilin variants. It is surprising, that all strains still appear to bind a common receptor, asialo-GM1. Here, we present the 1.63 A crystal structure of pilin from P. aeruginosa strain, PAK. The structure shows that the proposed receptor-binding site is formed, by two beta-turns that create a surface dominated by main-chain atoms., Receptor specificity could therefore be maintained, whilst allowing, side-chain variation, if the main-chain conformation is conserved. The, location of the binding site relative to the proposed packing of the pilus, fiber raises new issues and suggests that the current fiber model may have, to be reconsidered. Finally, the structure of the C-terminal, disulphide-bonded loop will provide the template for the structure-based, design of a consensus sequence vaccine.
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<StructureSection load='1dzo' size='340' side='right'caption='[[1dzo]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dzo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAK Pseudomonas aeruginosa PAK]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DZO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dzo OCA], [https://pdbe.org/1dzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dzo RCSB], [https://www.ebi.ac.uk/pdbsum/1dzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dzo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FMPA_PSEAI FMPA_PSEAI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dz/1dzo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dzo ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DZO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DZO OCA].
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*[[Pilin 3D structures|Pilin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Pseudomonas aeruginosa PAK pilin suggests a main-chain-dominated mode of receptor binding., Hazes B, Sastry PA, Hayakawa K, Read RJ, Irvin RT, J Mol Biol. 2000 Jun 16;299(4):1005-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10843854 10843854]
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Pseudomonas aeruginosa PAK]]
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[[Category: Single protein]]
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[[Category: Hazes B]]
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[[Category: Hazes, B.]]
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[[Category: Read RJ]]
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[[Category: Read, R.J.]]
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[[Category: adhesin]]
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[[Category: lectin]]
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[[Category: type iv pilin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:41:55 2007''
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Current revision

Truncated PAK pilin from Pseudomonas aeruginosa

PDB ID 1dzo

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