1e0c

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[[Image:1e0c.gif|left|200px]]<br /><applet load="1e0c" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e0c, resolution 1.8&Aring;" />
 
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'''SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII'''<br />
 
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==Overview==
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==SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII==
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Rhodanese is an ubiquitous enzyme that in vitro catalyses the transfer of, a sulfur atom from suitable donors to nucleophilic acceptors by way of a, double displacement mechanism. During the catalytic process the enzyme, cycles between a sulfur-free and a persulfide-containing form, via, formation of a persulfide linkage to a catalytic Cys residue. In the, nitrogen-fixing bacteria Azotobacter vinelandii the rhdA gene has been, identified and the encoded protein functionally characterized as a, rhodanese. The crystal structure of the A. vinelandii rhodanese has been, determined and refined at 1.8 A resolution in the sulfur-free and, persulfide-containing forms. Conservation of the overall three-dimensional, fold of bovine rhodanese is observed, with substantial modifications of, the protein structure in the proximity of the catalytic residue Cys230., Remarkably, the native enzyme is found as the Cys230-persulfide form; in, the sulfur-free state the catalytic Cys residue adopts two alternate, conformations, reflected by perturbation of the neighboring active-site, residues, which is associated with a partly reversible loss of, thiosulfate:cyanide sulfurtransferase activity. The catalytic mechanism of, A. vinelandii rhodanese relies primarily on the main-chain conformation of, the 230 to 235 active-site loop and on a surrounding strong positive, electrostatic field. Substrate recognition is based on residues which are, entirely different in the prokaryotic and eukaryotic enzymes. The, active-site loop of A. vinelandii rhodanese displays striking structural, similarity to the active-site loop of the similarly folded catalytic, domain of dual specific phosphatase Cdc25, suggesting a common, evolutionary origin of the two enzyme families.
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<StructureSection load='1e0c' size='340' side='right'caption='[[1e0c]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1e0c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E0C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e0c OCA], [https://pdbe.org/1e0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e0c RCSB], [https://www.ebi.ac.uk/pdbsum/1e0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e0c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THTR_AZOVI THTR_AZOVI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/1e0c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e0c ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1E0C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with SO4, MG and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thiosulfate_sulfurtransferase Thiosulfate sulfurtransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.1 2.8.1.1] Known structural/functional Site: <scene name='pdbsite=CSS:In Native Crystals, The Sg Atom Of Cys230 Forms A Persul ...'>CSS</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E0C OCA].
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*[[Sulfurtransferase|Sulfurtransferase]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of a sulfurtransferase from Azotobacter vinelandii highlights the evolutionary relationship between the rhodanese and phosphatase enzyme families., Bordo D, Deriu D, Colnaghi R, Carpen A, Pagani S, Bolognesi M, J Mol Biol. 2000 May 12;298(4):691-704. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10788330 10788330]
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[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Thiosulfate sulfurtransferase]]
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[[Category: Bolognesi M]]
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[[Category: Bolognesi, M.]]
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[[Category: Bordo D]]
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[[Category: Bordo, D.]]
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[[Category: Carpen A]]
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[[Category: Carpen, A.]]
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[[Category: Colnaghi R]]
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[[Category: Colnaghi, R.]]
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[[Category: Deriu D]]
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[[Category: Deriu, D.]]
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[[Category: Pagani S]]
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[[Category: Pagani, S.]]
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[[Category: EDO]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: sulfur metabolism]]
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[[Category: sulfurtransferase]]
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[[Category: thiosulfate:cyanide sulfurtransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:49:55 2007''
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Current revision

SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII

PDB ID 1e0c

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