This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1eaq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:00, 20 March 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_1eaq| PDB=1eaq | SCENE= }}
 
-
===THE RUNX1 RUNT DOMAIN AT 1.25A RESOLUTION: A STRUCTURAL SWITCH AND SPECIFICALLY BOUND CHLORIDE IONS MODULATE DNA BINDING===
 
-
{{ABSTRACT_PUBMED_12217689}}
 
-
==Disease==
+
==The RUNX1 Runt domain at 1.25A resolution: A structural switch and specifically bound chloride ions modulate DNA binding==
-
[[http://www.uniprot.org/uniprot/RUNX1_MOUSE RUNX1_MOUSE]] Note=Mice with an Runx1 lacking the DNA-binding region are found to die between embryonic days 11.5 to 12.5 due to hemorrhaging in the central nervous system. This hemorrhaging is preceded by necrosis and hematopoiesis is blocked.
+
<StructureSection load='1eaq' size='340' side='right'caption='[[1eaq]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1eaq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EAQ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eaq OCA], [https://pdbe.org/1eaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eaq RCSB], [https://www.ebi.ac.uk/pdbsum/1eaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eaq ProSAT]</span></td></tr>
 +
</table>
 +
== Disease ==
 +
[https://www.uniprot.org/uniprot/RUNX1_MOUSE RUNX1_MOUSE] Note=Mice with an Runx1 lacking the DNA-binding region are found to die between embryonic days 11.5 to 12.5 due to hemorrhaging in the central nervous system. This hemorrhaging is preceded by necrosis and hematopoiesis is blocked.
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RUNX1_MOUSE RUNX1_MOUSE] CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation (By similarity).<ref>PMID:8565077</ref> <ref>PMID:8622955</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/1eaq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eaq ConSurf].
 +
<div style="clear:both"></div>
-
==Function==
+
==See Also==
-
[[http://www.uniprot.org/uniprot/RUNX1_MOUSE RUNX1_MOUSE]] CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation (By similarity).<ref>PMID:8565077</ref><ref>PMID:8622955</ref>
+
*[[Core-binding factor|Core-binding factor]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
[[1eaq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAQ OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
[[Category: Large Structures]]
-
<ref group="xtra">PMID:012217689</ref><references group="xtra"/><references/>
+
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Backstrom, S.]]
+
[[Category: Backstrom S]]
-
[[Category: Grundstrom, C.]]
+
[[Category: Grundstrom C]]
-
[[Category: Grundstrom, T.]]
+
[[Category: Grundstrom T]]
-
[[Category: Hard, T.]]
+
[[Category: Hard T]]
-
[[Category: Sauer, U H.]]
+
[[Category: Sauer UH]]
-
[[Category: Wolf-Watz, M.]]
+
[[Category: Wolf-Watz M]]
-
[[Category: Acute myeloid leukemia]]
+
-
[[Category: Aml]]
+
-
[[Category: Chloride binding]]
+
-
[[Category: Ig fold]]
+
-
[[Category: Runt domain]]
+
-
[[Category: Runx1]]
+
-
[[Category: Transcription factor]]
+
-
[[Category: Transcription-dna complex]]
+
-
[[Category: Transcription/dna]]
+

Current revision

The RUNX1 Runt domain at 1.25A resolution: A structural switch and specifically bound chloride ions modulate DNA binding

PDB ID 1eaq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1eaq"
Personal tools