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1eb6

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[[Image:1eb6.gif|left|200px]]<br />
 
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<applet load="1eb6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eb6, resolution 1.00&Aring;" />
 
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'''DEUTEROLYSIN FROM ASPERGILLUS ORYZAE'''<br />
 
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==Overview==
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==Deuterolysin from Aspergillus oryzae==
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A data set from the metalloproteinase deuterolysin was collected at atomic, resolution (1.0 A) with synchrotron radiation. The high resolution allowed, the structure to be solved with the new direct-methods program ACORN using, the coordinates of the Zn atom as a starting point. The phases obtained, from ACORN were of sufficient quality to allow automated building to be, carried out in ARP/wARP. Minimal manual rebuilding of the model was, required and the structure determination was completed using the, maximum-likelihood refinement program REFMAC. The whole process, starting, from the processed and merged data and ending with a refined model, required less than 6 h of computational time.
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<StructureSection load='1eb6' size='340' side='right'caption='[[1eb6]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1eb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EB6 FirstGlance]. <br>
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1EB6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]] with ZN and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39]]. Structure known Active Site: ZN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB6 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eb6 OCA], [https://pdbe.org/1eb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eb6 RCSB], [https://www.ebi.ac.uk/pdbsum/1eb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eb6 ProSAT]</span></td></tr>
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A quick solution: ab initio structure determination of a 19 kDa metalloproteinase using ACORN., McAuley KE, Jia-Xing Y, Dodson EJ, Lehmbeck J, Ostergaard PR, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1571-8. Epub 2001, Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11679721 11679721]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NPII_ASPOR NPII_ASPOR] Metalloprotease that shows high activities on basic nuclear substrates such as histone and protamine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/1eb6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eb6 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
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[[Category: Deuterolysin]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dodson EJ]]
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[[Category: Dodson, E.J.]]
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[[Category: Jia-Xing Y]]
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[[Category: Jia-Xing, Y.]]
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[[Category: Lehmbeck J]]
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[[Category: Lehmbeck, J.]]
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[[Category: McAuley KE]]
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[[Category: Mcauley, K.E.]]
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[[Category: Ostergaard PR]]
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[[Category: Ostergaard, P.R.]]
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[[Category: Wilson KS]]
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[[Category: Wilson, K.S.]]
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[[Category: EDO]]
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[[Category: ZN]]
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[[Category: hydrolase]]
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[[Category: metalloproteinase]]
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[[Category: neutral protease ii]]
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[[Category: zinc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:10:04 2007''
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Current revision

Deuterolysin from Aspergillus oryzae

PDB ID 1eb6

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