This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ecz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:01, 20 March 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ecz.jpg|left|200px]]<br /><applet load="1ecz" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ecz, resolution 2.68&Aring;" />
 
-
'''PROTEASE INHIBITOR ECOTIN'''<br />
 
-
==Overview==
+
==PROTEASE INHIBITOR ECOTIN==
-
Ecotin, a homodimeric protein composed of 142 residue subunits, is a novel, serine protease inhibitor present in Escherichia coli. Its thermostability, and acid stability, as well as broad specificity toward proteases, make it, an interesting protein for structural characterization. Its structure in, the uncomplexed state, determined for two different crystalline, environments, allows a structural comparison of the free inhibitor with, that in complex with trypsin. Although there is no gross structural, rearrangement of ecotin when binding trypsin, the loops involved in, binding trypsin show relatively large shifts in atomic positions. The, inherent flexibility of the loops and the highly nonglobular shape are the, two features essential for its inhibitory function. An insight into the, understanding of the structural basis of thermostability and acid, stability of ecotin is also provided by the present structure.
+
<StructureSection load='1ecz' size='340' side='right'caption='[[1ecz]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ecz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ECZ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ecz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ecz OCA], [https://pdbe.org/1ecz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ecz RCSB], [https://www.ebi.ac.uk/pdbsum/1ecz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ecz ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ECOT_ECOLI ECOT_ECOLI] General inhibitor of pancreatic serine proteases: inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity.[HAMAP-Rule:MF_00706]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/1ecz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ecz ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1ECZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with BOG as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=A1:P1 Reactive Site Chain A'>A1</scene> and <scene name='pdbsite=B1:P1 Reactive Site Chain B'>B1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECZ OCA].
+
*[[Ecotin|Ecotin]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure analyses of uncomplexed ecotin in two crystal forms: implications for its function and stability., Shin DH, Song HK, Seong IS, Lee CS, Chung CH, Suh SW, Protein Sci. 1996 Nov;5(11):2236-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8931142 8931142]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Shin, D.H.]]
+
[[Category: Shin DH]]
-
[[Category: Suh, S.W.]]
+
[[Category: Suh SW]]
-
[[Category: BOG]]
+
-
[[Category: beta-sheet structure]]
+
-
[[Category: periplasmic]]
+
-
[[Category: serine protease inhibitor]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:11:35 2007''
+

Current revision

PROTEASE INHIBITOR ECOTIN

PDB ID 1ecz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ecz"
Personal tools