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1ed0

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[[Image:1ed0.gif|left|200px]]
 
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{{Structure
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==NMR structural determination of viscotoxin A3 from Viscum album L.==
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|PDB= 1ed0 |SIZE=350|CAPTION= <scene name='initialview01'>1ed0</scene>
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<StructureSection load='1ed0' size='340' side='right'caption='[[1ed0]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ed0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ED0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ED0 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ed0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ed0 OCA], [https://pdbe.org/1ed0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ed0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ed0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ed0 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1cbn|1cbn]], [[1cnr|1cnr]], [[1ccm|1ccm]], [[1ab1|1ab1]], [[2plh|2plh]], [[1bhp|1bhp]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ed0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ed0 OCA], [http://www.ebi.ac.uk/pdbsum/1ed0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ed0 RCSB]</span>
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[https://www.uniprot.org/uniprot/THN3_VISAL THN3_VISAL] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''NMR structural determination of viscotoxin A3 from Viscum album L.'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ed/1ed0_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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The high-resolution three-dimensional structure of the plant toxin viscotoxin A3, from Viscum album L., has been determined in solution by (1)H NMR spectroscopy at pH 3.6 and 12 degrees C (the structure has been deposited in the Protein Data Bank under the id. code 1ED0). Experimentally derived restraints including 734 interproton distances from nuclear Overhauser effect measurements, 22 hydrogen bonds, 32 &phi; angle restraints from J coupling measurements, together with three disulphide bridge constraints were used as input in restrained molecular dynamics, followed by minimization, using DYANA and Discover. Backbone and heavy atom root-mean-square deviations were 0.47+/-0.11 A (1 A=10(-10) m) and 0.85+/-0.13 A respectively. Viscotoxin A3 consists of two alpha-helices connected by a turn and a short stretch of antiparallel beta-sheet. This fold is similar to that found in other thionins, such as crambin, hordothionin-alpha and -beta, phoratoxin A and purothionin-alpha and -beta. The difference in the observed biological activity for thionins of known structure is discussed in terms of the differences in the calculated surface potential distribution, playing an important role in their function through disruption of cell membranes. In addition, the possible role in DNA binding of the helix-turn-helix motif of viscotoxin A3 is discussed.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ed0 ConSurf].
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1ED0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ED0 OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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NMR structural determination of viscotoxin A3 from Viscum album L., Romagnoli S, Ugolini R, Fogolari F, Schaller G, Urech K, Giannattasio M, Ragona L, Molinari H, Biochem J. 2000 Sep 1;350 Pt 2:569-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10947973 10947973]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Viscum album]]
[[Category: Viscum album]]
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[[Category: Fogolari, F.]]
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[[Category: Fogolari F]]
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[[Category: Giannattasio, M.]]
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[[Category: Giannattasio M]]
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[[Category: Molinari, H.]]
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[[Category: Molinari H]]
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[[Category: Ragona, L.]]
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[[Category: Ragona L]]
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[[Category: Romagnoli, S.]]
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[[Category: Romagnoli S]]
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[[Category: Schaller, G.]]
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[[Category: Schaller G]]
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[[Category: Ugolini, R.]]
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[[Category: Ugolini R]]
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[[Category: Urech, K.]]
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[[Category: Urech K]]
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[[Category: alpha-beta protein]]
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[[Category: amphipathic helix]]
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[[Category: concentric motif]]
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[[Category: helix-turn-helix]]
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[[Category: thionin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:00:06 2008''
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Current revision

NMR structural determination of viscotoxin A3 from Viscum album L.

PDB ID 1ed0

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