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1eg5

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[[Image:1eg5.gif|left|200px]]<br /><applet load="1eg5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eg5, resolution 2.00&Aring;" />
 
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'''NIFS-LIKE PROTEIN'''<br />
 
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==Overview==
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==NIFS-LIKE PROTEIN==
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NifS-like proteins are ubiquitous, homodimeric, proteins which belong to the alpha-family of pyridoxal-5'-phoshate dependent enzymes. They are proposed to donate elementary sulphur, generated from cysteine, via a cysteinepersulphide intermediate during iron sulphur cluster biosynthesis, an important albeit not well understood process. Here, we report on the crystal structure of a NifS-like protein from the hyperthermophilic bacterium Thermotoga maritima (tmNifS) at 2.0 A resolution. The tmNifS is structured into two domains, the larger bearing the pyridoxal-5'-phosphate-binding active site, the smaller hosting the active site cysteine in the middle of a highly flexible loop, 12 amino acid residues in length. Once charged with sulphur the loop could possibly deliver S(0) directly to regions far remote from the protein. Based on the three-dimensional structures of the native as well as the substrate complexed form and on spectrophotometric results, a mechanism of sulphur activation is proposed. The His99, which stacks on top of the pyridoxal-5'-phosphate co-factor, is assigned a crucial role during the catalytic cycle by acting as an acid-base catalyst and is believed to have a pK(a) value depending on the co-factor redox state.
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<StructureSection load='1eg5' size='340' side='right'caption='[[1eg5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1eg5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG5 FirstGlance]. <br>
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1EG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg5 OCA], [https://pdbe.org/1eg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg5 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg5 ProSAT]</span></td></tr>
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Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly., Kaiser JT, Clausen T, Bourenkow GP, Bartunik HD, Steinbacher S, Huber R, J Mol Biol. 2000 Mar 24;297(2):451-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10715213 10715213]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q9X218_THEMA Q9X218_THEMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1eg5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eg5 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Bartunik, H D.]]
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[[Category: Bartunik H-D]]
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[[Category: Bourenkow, G P.]]
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[[Category: Bourenkow GP]]
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[[Category: Clausen, T.]]
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[[Category: Clausen T]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Kaiser, J T.]]
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[[Category: Kaiser JT]]
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[[Category: Steinbacher, S.]]
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[[Category: Steinbacher S]]
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[[Category: PLP]]
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[[Category: SO4]]
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[[Category: c-s beta lyase]]
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[[Category: iron-sulfur-cluster synthesis]]
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[[Category: plp-dependent enzymes]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:28 2008''
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Current revision

NIFS-LIKE PROTEIN

PDB ID 1eg5

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