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1egn

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{{Seed}}
 
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[[Image:1egn.png|left|200px]]
 
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==CELLOBIOHYDROLASE CEL7A (E223S, A224H, L225V, T226A, D262G) MUTANT==
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The line below this paragraph, containing "STRUCTURE_1egn", creates the "Structure Box" on the page.
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<StructureSection load='1egn' size='340' side='right'caption='[[1egn]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1egn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EGN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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{{STRUCTURE_1egn| PDB=1egn | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1egn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1egn OCA], [https://pdbe.org/1egn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1egn RCSB], [https://www.ebi.ac.uk/pdbsum/1egn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1egn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1egn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1egn ConSurf].
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<div style="clear:both"></div>
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===CELLOBIOHYDROLASE CEL7A (E223S, A224H, L225V, T226A, D262G) MUTANT===
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==See Also==
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*[[Cellobiohydrolase 3D structures|Cellobiohydrolase 3D structures]]
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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The line below this paragraph, {{ABSTRACT_PUBMED_11336632}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 11336632 is the PubMed ID number.
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[[Category: Large Structures]]
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[[Category: Trichoderma reesei]]
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{{ABSTRACT_PUBMED_11336632}}
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[[Category: Harris M]]
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[[Category: Jones TA]]
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==About this Structure==
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[[Category: Stahlberg J]]
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1EGN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGN OCA].
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==Reference==
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Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant., Becker D, Braet C, Brumer H 3rd, Claeyssens M, Divne C, Fagerstrom BR, Harris M, Jones TA, Kleywegt GJ, Koivula A, Mahdi S, Piens K, Sinnott ML, Stahlberg J, Teeri TT, Underwood M, Wohlfahrt G, Biochem J. 2001 May 15;356(Pt 1):19-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11336632 11336632]
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[[Category: Cellulose 1,4-beta-cellobiosidase]]
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[[Category: Hypocrea jecorina]]
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[[Category: Single protein]]
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[[Category: Harris, M.]]
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[[Category: Jones, T A.]]
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[[Category: Stahlberg, J.]]
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[[Category: Cellulase]]
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[[Category: Cellulose degradation]]
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[[Category: Glycoprotein]]
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[[Category: Glycosidase]]
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[[Category: Glycosylated protein]]
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[[Category: Hydrolase]]
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[[Category: Ph-mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:40:09 2008''
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Current revision

CELLOBIOHYDROLASE CEL7A (E223S, A224H, L225V, T226A, D262G) MUTANT

PDB ID 1egn

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