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1eis

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[[Image:1eis.jpg|left|200px]]<br /><applet load="1eis" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eis, resolution 1.66&Aring;" />
 
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'''UDA UNCOMPLEXED FORM. CRYSTAL STRUCTURE OF URTICA DIOICA AGGLUTININ, A SUPERANTIGEN PRESENTED BY MHC MOLECULES OF CLASS I AND CLASS II'''<br />
 
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==Overview==
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==UDA UNCOMPLEXED FORM. CRYSTAL STRUCTURE OF URTICA DIOICA AGGLUTININ, A SUPERANTIGEN PRESENTED BY MHC MOLECULES OF CLASS I AND CLASS II==
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BACKGROUND: Urtica dioica agglutinin (UDA), a monomeric lectin extracted from stinging nettle rhizomes, is specific for saccharides containing N-acetylglucosamine (GlcNAc). The lectin behaves as a superantigen for murine T cells, inducing the exclusive proliferation of Vbeta8.3(+) lymphocytes. UDA is unique among known T cell superantigens because it can be presented by major histocompatibility complex (MHC) molecules of both class I and II. RESULTS: The crystal structure of UDA has been determined in the ligand-free state, and in complex with tri-acetylchitotriose and tetra-acetylchitotetraose at 1.66 A, 1.90 A and 1.40 A resolution, respectively. UDA comprises two hevein-like domains, each with a saccharide-binding site. A serine and three aromatic residues at each site form the principal contacts with the ligand. The N-terminal domain binding site can centre on any residue of a chito-oligosaccharide, whereas that of the C-terminal domain is specific for residues at the nonreducing terminus of the ligand. We have shown previously that oligomers of GlcNAc inhibit the superantigenic activity of UDA and that the lectin binds to glycans on the MHC molecule. We show that UDA also binds to glycans on the T cell receptor (TCR). CONCLUSIONS: The presence of two saccharide-binding sites observed in the structure of UDA suggests that its superantigenic properties arise from the simultaneous fixation of glycans on the TCR and MHC molecules of the T cell and antigen-presenting cell, respectively. The well defined spacing between the two binding sites of UDA is probably a key factor in determining the specificity for Vbeta8.3(+) lymphocytes.
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<StructureSection load='1eis' size='340' side='right'caption='[[1eis]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eis]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Urtica_dioica Urtica dioica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eis OCA], [https://pdbe.org/1eis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eis RCSB], [https://www.ebi.ac.uk/pdbsum/1eis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eis ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9SYR5_URTDI Q9SYR5_URTDI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eis_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eis ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Urtica_dioica Urtica dioica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIS OCA].
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Urtica dioica agglutinin, a superantigen presented by MHC molecules of class I and class II., Saul FA, Rovira P, Boulot G, Damme EJ, Peumans WJ, Truffa-Bachi P, Bentley GA, Structure. 2000 Jun 15;8(6):593-603. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10873861 10873861]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Urtica dioica]]
[[Category: Urtica dioica]]
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[[Category: Bentley, G A.]]
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[[Category: Bentley GA]]
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[[Category: Boulot, G.]]
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[[Category: Boulot G]]
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[[Category: Damme, E J.M Van.]]
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[[Category: Peumans WJ]]
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[[Category: Peumans, W J.]]
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[[Category: Rovira P]]
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[[Category: Rovira, P.]]
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[[Category: Saul FA]]
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[[Category: Saul, F A.]]
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[[Category: Truffa-Bachi P]]
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[[Category: Truffa-Bachi, P.]]
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[[Category: Van Damme EJM]]
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[[Category: hevein domain]]
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[[Category: lectin]]
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[[Category: superantigen]]
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[[Category: uda]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:13 2008''
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Current revision

UDA UNCOMPLEXED FORM. CRYSTAL STRUCTURE OF URTICA DIOICA AGGLUTININ, A SUPERANTIGEN PRESENTED BY MHC MOLECULES OF CLASS I AND CLASS II

PDB ID 1eis

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