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1elc

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[[Image:1elc.gif|left|200px]]
 
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{{Structure
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==Analogous inhibitors of elastase do not always bind analogously==
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|PDB= 1elc |SIZE=350|CAPTION= <scene name='initialview01'>1elc</scene>, resolution 1.75&Aring;
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<StructureSection load='1elc' size='340' side='right'caption='[[1elc]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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<table><tr><td colspan='2'>[[1elc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELC FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0Z3:6-AMMONIO-N-(TRIFLUOROACETYL)-L-NORLEUCYL-N-[4-(1-METHYLETHYL)PHENYL]-L-PHENYLALANINAMIDE'>0Z3</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elc OCA], [https://pdbe.org/1elc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elc RCSB], [https://www.ebi.ac.uk/pdbsum/1elc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG] Acts upon elastin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elc ConSurf].
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<div style="clear:both"></div>
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'''ANALOGOUS INHIBITORS OF ELASTASE DO NOT ALWAYS BIND ANALOGOUSLY'''
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==See Also==
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*[[Elastase 3D structures|Elastase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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It has been assumed that the structure of a single inhibitor complex is sufficient to define the available subsites of an enzyme that has a unique binding site and a uniquely defined mode for ligand binding--the specificity for these subsites can thus be probed by kinetic experiments. Elastase is an enzyme for which these traditional assumptions, which underlie such structural and kinetic studies, do not hold. Three new crystal structures of elastase complexed to chemically similar inhibitors with similar binding affinities reveal a diversity of binding modes as well as two new subsites on elastase. The existence of multiple binding sites and different binding modes for such similar inhibitors indicates that researchers must proceed with caution when using kinetics to map out protein subsites.
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[[Category: Large Structures]]
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==About this Structure==
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1ELC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELC OCA].
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==Reference==
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Analogous inhibitors of elastase do not always bind analogously., Mattos C, Rasmussen B, Ding X, Petsko GA, Ringe D, Nat Struct Biol. 1994 Jan;1(1):55-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7656008 7656008]
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[[Category: Pancreatic elastase]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Ding, X.]]
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[[Category: Ding X]]
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[[Category: Mattos, C.]]
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[[Category: Mattos C]]
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[[Category: Petsko, G A.]]
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[[Category: Petsko GA]]
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[[Category: Rasmussen, B.]]
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[[Category: Rasmussen B]]
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[[Category: Ringe, D.]]
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[[Category: Ringe D]]
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[[Category: CA]]
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[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:57:12 2008''
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Current revision

Analogous inhibitors of elastase do not always bind analogously

PDB ID 1elc

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