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1eqw

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(New page: 200px<br /><applet load="1eqw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eqw, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1eqw.jpg|left|200px]]<br /><applet load="1eqw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eqw, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF SALMONELLA TYPHIMURIUM CU,ZN SUPEROXIDE DISMUTASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF SALMONELLA TYPHIMURIUM CU,ZN SUPEROXIDE DISMUTASE==
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The functional and three-dimensional structural features of Cu,Zn, superoxide dismutase coded by the Salmonella typhimurium sodCI gene, have, been characterized. Measurements of the catalytic rate indicate that this, enzyme is the most efficient superoxide dismutase analyzed so far, a, feature that may be related to the exclusive association of the sodCI gene, with the most pathogenic Salmonella serotypes. The enzyme active-site, copper ion is highly accessible to external probes, as indicated by, quenching of the water proton relaxation rate upon addition of iodide. The, shape of the electron paramagnetic resonance spectrum is dependent on the, frozen or liquid state of the enzyme solution, suggesting relative, flexibility of the copper ion environment. The crystal structure (R-factor, 22.6%, at 2.3 A resolution) indicates that the dimeric enzyme adopts the, quaternary assembly typical of prokaryotic Cu,Zn superoxide dismutases., However, when compared to the structures of the homologous enzymes from, Photobacterium leiognathi and Actinobacillus pleuropneumoniae, the subunit, interface of Salmonella Cu,Zn superoxide dismutase shows substitution of, 11 out of 19 interface residues. As a consequence, the network of, structural water molecules that fill the dimer interface cavity is, structured differently from the other dimeric bacterial enzymes. The, crystallographic and functional characterization of this Salmonella Cu,Zn, superoxide dismutase indicates that structural variability and catalytic, efficiency are higher in prokaryotic than in the eukaryotic homologous, enzymes.
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<StructureSection load='1eqw' size='340' side='right'caption='[[1eqw]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eqw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eqw OCA], [https://pdbe.org/1eqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eqw RCSB], [https://www.ebi.ac.uk/pdbsum/1eqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eqw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SODC1_SALTY SODC1_SALTY] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/1eqw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eqw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EQW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with ZN and CU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EQW OCA].
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*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Functional and crystallographic characterization of Salmonella typhimurium Cu,Zn superoxide dismutase coded by the sodCI virulence gene., Pesce A, Battistoni A, Stroppolo ME, Polizio F, Nardini M, Kroll JS, Langford PR, O'Neill P, Sette M, Desideri A, Bolognesi M, J Mol Biol. 2000 Sep 15;302(2):465-78. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10970746 10970746]
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[[Category: Large Structures]]
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[[Category: Salmonella typhimurium]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Single protein]]
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[[Category: Battistoni A]]
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[[Category: Superoxide dismutase]]
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[[Category: Bolognesi M]]
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[[Category: Battistoni, A.]]
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[[Category: Desideri A]]
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[[Category: Bolognesi, M.]]
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[[Category: Kroll JS]]
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[[Category: Desideri, A.]]
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[[Category: Langford PR]]
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[[Category: Kroll, J.S.]]
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[[Category: Nardini M]]
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[[Category: Langford, P.R.]]
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[[Category: O'Neill P]]
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[[Category: Nardini, M.]]
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[[Category: Pesce A]]
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[[Category: Neill, P.O.]]
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[[Category: Polizio F]]
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[[Category: Pesce, A.]]
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[[Category: Sette M]]
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[[Category: Polizio, F.]]
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[[Category: Stroppolo ME]]
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[[Category: Sette, M.]]
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[[Category: Stroppolo, M.E.]]
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[[Category: CU]]
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[[Category: ZN]]
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[[Category: greek key b-barrel]]
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[[Category: superoxide dismutase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:14:23 2007''
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Current revision

CRYSTAL STRUCTURE OF SALMONELLA TYPHIMURIUM CU,ZN SUPEROXIDE DISMUTASE

PDB ID 1eqw

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