This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1euu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:06, 20 March 2024) (edit) (undo)
 
(21 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1euu.gif|left|200px]]<br />
 
-
<applet load="1euu" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1euu, resolution 2.5&Aring;" />
 
-
'''SIALIDASE OR NEURAMINIDASE, LARGE 68KD FORM'''<br />
 
-
==Overview==
+
==SIALIDASE OR NEURAMINIDASE, LARGE 68KD FORM==
-
BACKGROUND: Sialidases, or neuraminidases, have been implicated in the, pathogenesis of many diseases, but are also produced by many, non-pathogenic bacteria. Bacterial sialidases are very variable in size, often possessing domains in addition to the catalytic domain. The, sialidase from the non-pathogenic soil bacterium Micromonospora, viridifaciens is secreted in two forms with molecular weights of 41 kDa or, 68 kDa, depending on the nature of the carbohydrate used to induce, expression. RESULTS: We report here the X-ray crystal structures of the 41, kDa and 68 kDa forms of the sialidase from M. viridifaciens at 1.8 A and, 2.5 A resolution respectively. In addition, we report a complex of the 41, kDa form with an inhibitor at 2.0 A resolution, and a complex of the 68, kDa form with ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8591030 (full description)]]
+
<StructureSection load='1euu' size='340' side='right'caption='[[1euu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1euu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_viridifaciens Micromonospora viridifaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EUU FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1euu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1euu OCA], [https://pdbe.org/1euu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1euu RCSB], [https://www.ebi.ac.uk/pdbsum/1euu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1euu ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NANH_MICVI NANH_MICVI] To release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1euu_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1euu ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1EUU is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Micromonospora_viridifaciens Micromonospora viridifaciens]] with GAL and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EUU OCA]].
+
*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll., Gaskell A, Crennell S, Taylor G, Structure. 1995 Nov 15;3(11):1197-205. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8591030 8591030]
+
[[Category: Large Structures]]
-
[[Category: Exo-alpha-sialidase]]
+
[[Category: Micromonospora viridifaciens]]
[[Category: Micromonospora viridifaciens]]
-
[[Category: Single protein]]
+
[[Category: Crennell SJ]]
-
[[Category: Crennell, S.J.]]
+
[[Category: Gaskell A]]
-
[[Category: Gaskell, A.]]
+
[[Category: Taylor GL]]
-
[[Category: Taylor, G.L.]]
+
-
[[Category: GAL]]
+
-
[[Category: NA]]
+
-
[[Category: glycosidase]]
+
-
[[Category: hydrolase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:57:17 2007''
+

Current revision

SIALIDASE OR NEURAMINIDASE, LARGE 68KD FORM

PDB ID 1euu

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1euu"
Personal tools