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1f32
From Proteopedia
(Difference between revisions)
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<StructureSection load='1f32' size='340' side='right'caption='[[1f32]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='1f32' size='340' side='right'caption='[[1f32]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1f32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1f32]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F32 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f32 OCA], [https://pdbe.org/1f32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f32 RCSB], [https://www.ebi.ac.uk/pdbsum/1f32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f32 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f32 OCA], [https://pdbe.org/1f32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f32 RCSB], [https://www.ebi.ac.uk/pdbsum/1f32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f32 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/API3_ASCSU API3_ASCSU] This is an inhibitor of the aspartic protease pepsin. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f32 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f32 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The three-dimensional structures of pepsin inhibitor-3 (PI-3) from Ascaris suum and of the complex between PI-3 and porcine pepsin at 1. 75 A and 2.45 A resolution, respectively, have revealed the mechanism of aspartic protease inhibition by this unique inhibitor. PI-3 has a new fold consisting of two domains, each comprising an antiparallel beta-sheet flanked by an alpha-helix. In the enzyme-inhibitor complex, the N-terminal beta-strand of PI-3 pairs with one strand of the 'active site flap' (residues 70-82) of pepsin, thus forming an eight-stranded beta-sheet that spans the two proteins. PI-3 has a novel mode of inhibition, using its N-terminal residues to occupy and therefore block the first three binding pockets in pepsin for substrate residues C-terminal to the scissile bond (S1'-S3'). The molecular structure of the pepsin-PI-3 complex suggests new avenues for the rational design of proteinaceous aspartic proteinase inhibitors. | ||
| - | |||
| - | Structural basis for the inhibition of porcine pepsin by Ascaris pepsin inhibitor-3.,Ng KK, Petersen JF, Cherney MM, Garen C, Zalatoris JJ, Rao-Naik C, Dunn BM, Martzen MR, Peanasky RJ, James MN Nat Struct Biol. 2000 Aug;7(8):653-7. PMID:10932249<ref>PMID:10932249</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1f32" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Ascaris suum]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cherney | + | [[Category: Cherney MM]] |
| - | [[Category: Garen | + | [[Category: Garen C]] |
| - | [[Category: James | + | [[Category: James MN]] |
| - | [[Category: Ng | + | [[Category: Ng KK]] |
| - | [[Category: Petersen | + | [[Category: Petersen JF]] |
| - | + | ||
| - | + | ||
Current revision
CRYSTAL STRUCTURE OF ASCARIS PEPSIN INHIBITOR-3
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Categories: Ascaris suum | Large Structures | Cherney MM | Garen C | James MN | Ng KK | Petersen JF
