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1f8q

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[[Image:1f8q.gif|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF ALPHA-MOMORCHARIN IN ACETONITRILE-WATER MIXTURE==
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|PDB= 1f8q |SIZE=350|CAPTION= <scene name='initialview01'>1f8q</scene>, resolution 2.20&Aring;
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<StructureSection load='1f8q' size='340' side='right'caption='[[1f8q]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PTD:PENTANEDIAL'>PTD</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>
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<table><tr><td colspan='2'>[[1f8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F8Q FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PTD:PENTANEDIAL'>PTD</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8q OCA], [https://pdbe.org/1f8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f8q RCSB], [https://www.ebi.ac.uk/pdbsum/1f8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f8q ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1mri|1mri]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8q OCA], [http://www.ebi.ac.uk/pdbsum/1f8q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f8q RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/RIP1_MOMCH RIP1_MOMCH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/1f8q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f8q ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF ALPHA-MOMORCHARIN IN ACETONITRILE-WATER MIXTURE'''
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==See Also==
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Crystals of alpha-momorcharin (MMC) were cross-linked and soaked in an 80% acetonitrile--water mixture and X-ray data were collected to 2.2 A resolution. MMC is a ribosome-inactivating protein with a sugar chain on Asn-227. In previous studies, the whole conformation of the sugar chain could not be obtained in the aqueous system. Here the structure of MMC in a low water system is shown to be similar to the native one, but the sugar chain on Asn-227 is defined by the electron density map. Several oxygen atoms of the oligosaccharide formed intramolecular hydrogen bonds to the protein moiety. The conformation of the residues in the active center is similar to that in the aqueous system. Our results show conformational alteration of the tetrasaccharide of MMC in organic media. They indicate that the oligosaccharides are more rigid in organic solvents. X-ray determination in organic media may be used to solve some structures of oligosaccharides linked to glycoproteins.
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[[Category: Large Structures]]
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==About this Structure==
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1F8Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F8Q OCA].
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==Reference==
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Crystal structure of alpha-momorcharin in 80% acetonitrile--water mixture., Zhu G, Huang Q, Qian M, Tang Y, Biochim Biophys Acta. 2001 Jul 9;1548(1):152-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11451448 11451448]
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[[Category: Momordica charantia]]
[[Category: Momordica charantia]]
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[[Category: Single protein]]
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[[Category: Huang Q]]
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[[Category: rRNA N-glycosylase]]
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[[Category: Qian M]]
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[[Category: Huang, Q.]]
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[[Category: Tang Y]]
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[[Category: Qian, M.]]
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[[Category: Zhu G]]
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[[Category: Tang, Y.]]
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[[Category: Zhu, G.]]
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[[Category: momorcharin]]
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[[Category: organic slovent]]
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[[Category: ribosome-inactivating protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:18:02 2008''
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Current revision

CRYSTAL STRUCTURE OF ALPHA-MOMORCHARIN IN ACETONITRILE-WATER MIXTURE

PDB ID 1f8q

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