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1fat

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[[Image:1fat.gif|left|200px]]<br />
 
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<applet load="1fat" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fat, resolution 2.8&Aring;" />
 
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'''PHYTOHEMAGGLUTININ-L'''<br />
 
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==Overview==
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==PHYTOHEMAGGLUTININ-L==
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The structure of phytohemagglutinin-L (PHA-L), a leucoagglutinating seed, lectin from Phaseolus vulgaris, has been solved with molecular replacement, using the coordinates of lentil lectin as model, and refined at a, resolution of 2.8 A. The final R-factor of the structure is 20.0%. The, quaternary structure of the PHA-L tetramer differs from the structures of, the concanavalin A and peanut lectin tetramers, but resembles the, structure of the soybean agglutinin tetramer. PHA-L consists of two, canonical legume lectin dimers that pack together through the formation of, a close contact between two beta-strands. Of the two covalently bound, oligosaccharides per monomer, only one GlcNAc residue per monomer is, visible in the electron density. In this article we describe the structure, of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8702788 (full description)]]
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<StructureSection load='1fat' size='340' side='right'caption='[[1fat]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fat]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FAT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fat OCA], [https://pdbe.org/1fat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fat RCSB], [https://www.ebi.ac.uk/pdbsum/1fat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fat ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHAL_PHAVU PHAL_PHAVU] This insecticidal carbohydrate-binding lectin is toxic for the cowpea weevil.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/1fat_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fat ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FAT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]] with NAG, MN and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: CAA, CAB, CAC, CAD, MNA, MNB, MNC and MND. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FAT OCA]].
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*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystallographic structure of phytohemagglutinin-L., Hamelryck TW, Dao-Thi MH, Poortmans F, Chrispeels MJ, Wyns L, Loris R, J Biol Chem. 1996 Aug 23;271(34):20479-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8702788 8702788]
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[[Category: Large Structures]]
[[Category: Phaseolus vulgaris]]
[[Category: Phaseolus vulgaris]]
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[[Category: Single protein]]
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[[Category: Hamelryck T]]
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[[Category: Hamelryck, T.]]
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[[Category: Loris R]]
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[[Category: Loris, R.]]
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[[Category: CA]]
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[[Category: MN]]
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[[Category: NAG]]
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[[Category: glycoprotein]]
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[[Category: lectin]]
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[[Category: plant defense protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:11:50 2007''
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Current revision

PHYTOHEMAGGLUTININ-L

PDB ID 1fat

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