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1fgv

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(New page: 200px<br /> <applet load="1fgv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fgv, resolution 1.9&Aring;" /> '''X-RAY STRUCTURES OF ...)
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[[Image:1fgv.gif|left|200px]]<br />
 
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<applet load="1fgv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fgv, resolution 1.9&Aring;" />
 
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'''X-RAY STRUCTURES OF FRAGMENTS FROM BINDING AND NONBINDING VERSIONS OF A HUMANIZED ANTI-CD18 ANTIBODY: STRUCTURAL INDICATIONS OF THE KEY ROLE OF VH RESIDUES 59 TO 65'''<br />
 
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==Overview==
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==X-RAY STRUCTURES OF FRAGMENTS FROM BINDING AND NONBINDING VERSIONS OF A HUMANIZED ANTI-CD18 ANTIBODY: STRUCTURAL INDICATIONS OF THE KEY ROLE OF VH RESIDUES 59 TO 65==
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X-ray crystal structures of fragments from two different humanized, anti-CD18 antibodies are reported. The Fv fragment of the nonbinding, version has been refined in space group C2 with a = 64.2 A, b = 61.3 A, c, = 51.8 A, and beta = 99 degrees to an R-value of 18.0% at 1.9 A, and the, Fab fragment of the tight-binding version has been refined in space group, P3 with a = 101. A and c = 45.5 A to an R-value of 17.8% at 3.0 A, resolution. The very large difference in their binding affinity (&gt;, 1000-fold) is attributed to large and local structural differences in the, C-terminal part of CDR-H2, and from this we conclude there is direct, contact between this region and antigen when they combine. X-ray, structures of antibody-antigen complexes available in the literature have, yet to show this part of CDR-H2 in contact with antigen, despite its, hypervariable sequence. Implications of this result for antibody, humanization are discussed.
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<StructureSection load='1fgv' size='340' side='right'caption='[[1fgv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1fgv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FGV FirstGlance]. <br>
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1FGV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FGV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fgv OCA], [https://pdbe.org/1fgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fgv RCSB], [https://www.ebi.ac.uk/pdbsum/1fgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fgv ProSAT]</span></td></tr>
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==Reference==
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</table>
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X-ray structures of fragments from binding and nonbinding versions of a humanized anti-CD18 antibody: structural indications of the key role of VH residues 59 to 65., Eigenbrot C, Gonzalez T, Mayeda J, Carter P, Werther W, Hotaling T, Fox J, Kessler J, Proteins. 1994 Jan;18(1):49-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7908437 7908437]
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== Evolutionary Conservation ==
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[[Category: Single protein]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Eigenbrot, C.]]
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Check<jmol>
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[[Category: Kessler, J.]]
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<jmolCheckbox>
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[[Category: immunoglobulin]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/1fgv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:30:09 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fgv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Eigenbrot C]]
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[[Category: Kessler J]]

Current revision

X-RAY STRUCTURES OF FRAGMENTS FROM BINDING AND NONBINDING VERSIONS OF A HUMANIZED ANTI-CD18 ANTIBODY: STRUCTURAL INDICATIONS OF THE KEY ROLE OF VH RESIDUES 59 TO 65

PDB ID 1fgv

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