Cephalosporin acylase

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(New page: '''Cephalosporin acylase''' (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA). CSA contains 2 non-identical subunits. CSA is used commerci...)
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<StructureSection load='1jw0' size='350' side='right' caption='Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry [[1jw0]])' scene='52/525143/Cv/1'>
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'''Cephalosporin acylase''' (CSA) catalyzes the conversion of [[cephalosporin]] C (CPC) to 7-amino-cephalosporanic acid (7-ACA).<ref>PMID:11080627</ref>
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'''Cephalosporin acylase''' (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA). CSA contains 2 non-identical subunits. CSA is used commercially for production of semi-synthetic cephalosporin antibiotics. The active site pocket of CSA is similar to that of penicillin acylase. CSA precursor is cleaved at 2 sites forming the active heterodimer.
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== Relevance ==
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CSA is used commercially for production of semi-synthetic cephalosporin antibiotics.
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== Structural highlights ==
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<scene name='52/525143/Cv/2'>CSA contains 2 non-identical subunits</scene>. The <scene name='52/525143/Cv/4'>active site pocket</scene> (PDB entry [[1jw0]])<ref>PMID:11755403</ref> of CSA is similar to that of penicillin acylase. Water molecules are shoen as red spheres. CSA precursor is cleaved at 2 sites forming the active heterodimer.
==3D structures of cephalosporin acylase==
==3D structures of cephalosporin acylase==
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[[Cephalosporin acylase 3D structures]]
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[[2nlz]] - CSA – ''Bacillus halodurans''<br />
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[[1fm2]] - BdCSA – ''Brevundimonas diminuta''<br />
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[[1gk0]], [[1gk1]], [[1ghd]] - PsCSA – ''Pseudomonas''<br />
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[[2ae3]], [[2ae5]] - PsCSA α + β (mutant) subunits<br />
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[[2ae4]] - PsCSA α (mutant) + β subunits<br />
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[[1keh]] - BdCSA precursor (mutant)
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===Cephalosporin acylase complex===
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</StructureSection>
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[[1jvz]] – BdCSA + glutaryl-7-ACA<br />
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== References ==
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[[1jw0]] - BdCSA + glutarate
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<references/>
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[[Category:Topic Page]]

Current revision

Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry 1jw0)

Drag the structure with the mouse to rotate

References

  1. ↑ Kim Y, Yoon K, Khang Y, Turley S, Hol WG. The 2.0 A crystal structure of cephalosporin acylase. Structure. 2000 Oct 15;8(10):1059-68. PMID:11080627
  2. ↑ Kim Y, Hol WG. Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity. Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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