Cephalosporin acylase

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{{STRUCTURE_1jvz| PDB=1jvz | SIZE=400| SCENE= |right|CAPTION=Cephalosporin acylase α subunit (grey) and β subunit (green) complex with glutaryl 7-amino-cephalosporanic acid, [[1jvz]] }}
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<StructureSection load='1jw0' size='350' side='right' caption='Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry [[1jw0]])' scene='52/525143/Cv/1'>
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'''Cephalosporin acylase''' (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA). CSA contains 2 non-identical subunits. CSA is used commercially for production of semi-synthetic cephalosporin antibiotics. The active site pocket of CSA is similar to that of penicillin acylase. CSA precursor is cleaved at 2 sites forming the active heterodimer.
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'''Cephalosporin acylase''' (CSA) catalyzes the conversion of [[cephalosporin]] C (CPC) to 7-amino-cephalosporanic acid (7-ACA).<ref>PMID:11080627</ref>
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==3D structures of cephalosporin acylase==
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== Relevance ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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CSA is used commercially for production of semi-synthetic cephalosporin antibiotics.
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Cephalosporin acylase
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== Structural highlights ==
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**[[2nlz]] - CSA – ''Bacillus halodurans''<br />
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<scene name='52/525143/Cv/2'>CSA contains 2 non-identical subunits</scene>. The <scene name='52/525143/Cv/4'>active site pocket</scene> (PDB entry [[1jw0]])<ref>PMID:11755403</ref> of CSA is similar to that of penicillin acylase. Water molecules are shoen as red spheres. CSA precursor is cleaved at 2 sites forming the active heterodimer.
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**[[1fm2]] - BdCSA – ''Brevundimonas diminuta''<br />
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**[[1gk0]], [[1gk1]], [[1ghd]], [[4hsr]], [[4hst]] - PsCSA – ''Pseudomonas''<br />
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==3D structures of cephalosporin acylase==
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**[[2ae3]], [[2ae5]], [[3jtq]], [[2adv]] - PsCSA α + β (mutant) subunits<br />
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[[Cephalosporin acylase 3D structures]]
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**[[2ae4]], [[4e55]], [[4e56]], [[4e57]], [[3jtr]] - PsCSA α (mutant) + β subunits<br />
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**[[1keh]] - BdCSA precursor (mutant)
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*Cephalosporin acylase complex
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</StructureSection>
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**[[1jvz]] – BdCSA + glutaryl-7-ACA<br />
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== References ==
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**[[1jw0]] - BdCSA + glutarate
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<references/>
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}}
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[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry 1jw0)

Drag the structure with the mouse to rotate

References

  1. ↑ Kim Y, Yoon K, Khang Y, Turley S, Hol WG. The 2.0 A crystal structure of cephalosporin acylase. Structure. 2000 Oct 15;8(10):1059-68. PMID:11080627
  2. ↑ Kim Y, Hol WG. Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity. Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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