Cephalosporin acylase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:15, 26 March 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
 +
<StructureSection load='1jw0' size='350' side='right' caption='Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry [[1jw0]])' scene='52/525143/Cv/1'>
-
<StructureSection load='1jvz' size='350' side='right' caption='Cephalosporin acylase α subunit (grey) and β subunit (green) complex with glutaryl 7-amino-cephalosporanic acid (PDB entry [[1jvz]])' scene=''>
+
'''Cephalosporin acylase''' (CSA) catalyzes the conversion of [[cephalosporin]] C (CPC) to 7-amino-cephalosporanic acid (7-ACA).<ref>PMID:11080627</ref>
-
 
+
-
'''Cephalosporin acylase''' (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA).
+
== Relevance ==
== Relevance ==
Line 10: Line 9:
== Structural highlights ==
== Structural highlights ==
-
CSA contains 2 non-identical subunits. The active site pocket of CSA is similar to that of penicillin acylase. CSA precursor is cleaved at 2 sites forming the active heterodimer.
+
<scene name='52/525143/Cv/2'>CSA contains 2 non-identical subunits</scene>. The <scene name='52/525143/Cv/4'>active site pocket</scene> (PDB entry [[1jw0]])<ref>PMID:11755403</ref> of CSA is similar to that of penicillin acylase. Water molecules are shoen as red spheres. CSA precursor is cleaved at 2 sites forming the active heterodimer.
-
</StructureSection>
 
==3D structures of cephalosporin acylase==
==3D structures of cephalosporin acylase==
 +
[[Cephalosporin acylase 3D structures]]
-
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+
</StructureSection>
-
{{#tree:id=OrganizedByTopic|openlevels=0|
+
-
 
+
-
*Cephalosporin acylase
+
-
+
-
**[[2nlz]] - CSA – ''Bacillus halodurans''<br />
+
-
**[[1fm2]] - BdCSA – ''Brevundimonas diminuta''<br />
+
-
**[[1gk0]], [[1gk1]], [[1ghd]], [[4hsr]], [[4hst]] - PsCSA – ''Pseudomonas''<br />
+
-
**[[2ae3]], [[2ae5]], [[3jtq]], [[2adv]] - PsCSA α + β (mutant) subunits<br />
+
-
**[[2ae4]], [[4e55]], [[4e56]], [[4e57]], [[3jtr]] - PsCSA α (mutant) + β subunits<br />
+
-
**[[1keh]] - BdCSA precursor (mutant)
+
-
 
+
-
*Cephalosporin acylase complex
+
-
**[[1jvz]] – BdCSA + glutaryl-7-ACA<br />
 
-
**[[1jw0]] - BdCSA + glutarate
 
-
}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Cephalosporin acylase α subunit (magenta) and β subunit (green) complex with glutarate (PDB entry 1jw0)

Drag the structure with the mouse to rotate

References

  1. ↑ Kim Y, Yoon K, Khang Y, Turley S, Hol WG. The 2.0 A crystal structure of cephalosporin acylase. Structure. 2000 Oct 15;8(10):1059-68. PMID:11080627
  2. ↑ Kim Y, Hol WG. Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity. Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

Retrieved from "http://52.214.119.220/wiki/index.php/Cephalosporin_acylase"
Personal tools