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5zt3

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==Crystal structure of WA352 from Oryza sativa==
==Crystal structure of WA352 from Oryza sativa==
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<StructureSection load='5zt3' size='340' side='right' caption='[[5zt3]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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<StructureSection load='5zt3' size='340' side='right'caption='[[5zt3]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5zt3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZT3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5zt3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Indica_Group Oryza sativa Indica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZT3 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zt3 OCA], [http://pdbe.org/5zt3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zt3 RCSB], [http://www.ebi.ac.uk/pdbsum/5zt3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zt3 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.304&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zt3 OCA], [https://pdbe.org/5zt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zt3 RCSB], [https://www.ebi.ac.uk/pdbsum/5zt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zt3 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/M1SWB3_ORYSI M1SWB3_ORYSI]
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Plant cytoplasmic male sterility (CMS) is an important phenomenon and is widely utilized in hybrid crop breeding. The Wild Abortive CMS (CMS-WA), a well-known CMS type, has been successfully applied in the commercial production of hybrid rice seeds for more than 40 years. The CMS-WA causal gene WA352 encodes a novel transmenbrane protein and the interacts with the mitochondrial copper chaperone COX11, triggering reactive oxygen species production and resulting in male sterility in CMS-WA lines. However, the structure of WA352 is currently unknown, and the structural mechanism whereby WA352 perturbs COX11 function to cause CMS remains largely unknown. Here, we report the crystal structure of the C-terminal functional domain of WA352 at 1.3A resolution. This functional domain, consisting of five alpha helices, is spindle-shaped with a length of 42A, and a diameter of 28A. Notably, the absence of any structural similarity to a known protein structure suggests that the WA352 functional domain is a novel fold. In addition, surface conservation analysis and structural modeling of the WA352-COX11 complex revealed details about the WA352-COX11 interaction. Further structural analysis suggested that the WA352-COX11 interaction blocks the copper ion transportation activity of COX11, which is essential for the assembly of cytochrome c oxidase, resulting in male sterility in CMS-WA lines. Our study paves the way toward structural determination of the WA352-COX11 complex and provides new insight into the mechanism of plant CMS.
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Crystal structure of WA352 provides insight into cytoplasmic male sterility in rice.,Wang X, Guan Z, Gong Z, Yan J, Yang G, Liu YG, Yin P Biochem Biophys Res Commun. 2018 May 22. pii: S0006-291X(18)31136-7. doi:, 10.1016/j.bbrc.2018.05.079. PMID:29775612<ref>PMID:29775612</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5zt3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Guan, Z]]
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[[Category: Large Structures]]
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[[Category: Wang, X]]
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[[Category: Oryza sativa Indica Group]]
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[[Category: Yin, P]]
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[[Category: Guan Z]]
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[[Category: Cytoplasmic male sterility protein]]
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[[Category: Wang X]]
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[[Category: Plant protein]]
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[[Category: Yin P]]

Current revision

Crystal structure of WA352 from Oryza sativa

PDB ID 5zt3

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