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6ifs

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==KsgA from Bacillus subtilis 168==
==KsgA from Bacillus subtilis 168==
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<StructureSection load='6ifs' size='340' side='right' caption='[[6ifs]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
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<StructureSection load='6ifs' size='340' side='right'caption='[[6ifs]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ifs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IFS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ifs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IFS FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rsmA, ksgA, BSU00420 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/16S_rRNA_(adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.182 2.1.1.182] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ifs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifs OCA], [https://pdbe.org/6ifs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ifs RCSB], [https://www.ebi.ac.uk/pdbsum/6ifs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifs ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ifs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ifs OCA], [http://pdbe.org/6ifs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ifs RCSB], [http://www.ebi.ac.uk/pdbsum/6ifs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ifs ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RSMA_BACSU RSMA_BACSU]] Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.[HAMAP-Rule:MF_00607]
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[https://www.uniprot.org/uniprot/RSMA_BACSU RSMA_BACSU] Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.[HAMAP-Rule:MF_00607]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Post-translational methylation of ribosomal RNA at select positions is a prevalent resistance mechanism adopted by pathogens. In this work, KsgA, a housekeeping ribosomal methyltransferase (rMtase) involved in ribosome biogenesis, was exploited as a model system to delineate the specific targeting determinants that impart substrate specificity to rMtases. With a combination of evolutionary and structure-guided approaches, a set of chimeras were created that altered the targeting specificity of KsgA such that it acted similarly to erythromycin-resistant methyltransferases (Erms), rMtases found in multidrug-resistant pathogens. The results revealed that specific loop embellishments on the basic Rossmann fold are key determinants in the selection of the cognate RNA. Moreover, in vivo studies confirmed that chimeric constructs are competent in imparting macrolide resistance. This work explores the factors that govern the emergence of resistance and paves the way for the design of specific inhibitors useful in reversing antibiotic resistance.
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Deciphering Determinants in Ribosomal Methyltransferases that Confer Antimicrobial Resistance.,Bhujbalrao R, Anand R J Am Chem Soc. 2019 Jan 9. doi: 10.1021/jacs.8b10277. PMID:30624914<ref>PMID:30624914</ref>
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==See Also==
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*[[Adenosine dimethyltransferase 3D structures|Adenosine dimethyltransferase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ifs" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacsu]]
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[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Anand, R]]
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[[Category: Large Structures]]
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[[Category: Bhujbalrao, R]]
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[[Category: Anand R]]
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[[Category: Ksga]]
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[[Category: Bhujbalrao R]]
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[[Category: Resistance]]
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[[Category: Rossmann fold]]
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[[Category: Transferase]]
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Current revision

KsgA from Bacillus subtilis 168

PDB ID 6ifs

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