6j4n

<table><tr><td colspan='2'>[[6j4n]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Aciba Aciba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J4N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J4N FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j4n OCA], [http://pdbe.org/6j4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j4n RCSB], [http://www.ebi.ac.uk/pdbsum/6j4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j4n ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Metallo-beta-lactamases (MBLs) are present in major Gram-negative pathogens and environmental species, and pose great health risks because of their ability to hydrolyze the beta-lactam rings of antibiotics such as carbapenems. PNGM-1 was the first reported case of a subclass B3 MBL protein that was identified from a metagenomic library from deep-sea sediments that predate the antibiotic era. In this study, PNGM-1 was overexpressed, purified and crystallized. Crystals of native and selenomethionine-substituted PNGM-1 diffracted to 2.10 and 2.30 A resolution, respectively. Both the native and the selenomethionine-labelled PNGM-1 crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 122, b = 83, c = 163 A, beta = 110 degrees . Matthews coefficient (VM) calculations suggested the presence of 6-10 molecules in the asymmetric unit, corresponding to a solvent content of approximately 31-58%. Structure determination is currently in progress.

The novel metallo-beta-lactamase PNGM-1 from a deep-sea sediment metagenome: crystallization and X-ray crystallographic analysis.,Park KS, Hong MK, Jeon JW, Kim JH, Jeon JH, Lee JH, Kim TY, Karim AM, Malik SK, Kang LW, Lee SH Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):644-649. doi:, 10.1107/S2053230X18012268. Epub 2018 Sep 19. PMID:30279316<ref>PMID:30279316</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6j4n" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Aciba]]

[[Category: Hong, M K]]

[[Category: Jeon, J H]]

[[Category: Kang, L W]]

[[Category: Lee, J H]]

[[Category: Lee, S H]]

[[Category: Park, K S]]

[[Category: Park, Y S]]

[[Category: Hydrolase]]

[[Category: Zinc binding motif]]