6j6k

<table><tr><td colspan='2'>[[6j6k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J6K FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6k OCA], [http://pdbe.org/6j6k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j6k RCSB], [http://www.ebi.ac.uk/pdbsum/6j6k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6k ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 A resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.

 

== References ==

[[Category: Fan, X]]

[[Category: Lei, J L]]

[[Category: Wang, H W]]

[[Category: Wang, J]]

[[Category: Streptavidin]]