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6k9f

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Current revision (10:41, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6k9f' size='340' side='right'caption='[[6k9f]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
<StructureSection load='6k9f' size='340' side='right'caption='[[6k9f]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6k9f]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K9F FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6k9f]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K9F FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CARD8, KIAA0955, NDPP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k9f OCA], [https://pdbe.org/6k9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k9f RCSB], [https://www.ebi.ac.uk/pdbsum/6k9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k9f ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k9f OCA], [https://pdbe.org/6k9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k9f RCSB], [https://www.ebi.ac.uk/pdbsum/6k9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k9f ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/CARD8_HUMAN CARD8_HUMAN]] Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
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[https://www.uniprot.org/uniprot/CARD8_HUMAN CARD8_HUMAN] Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nod-like receptor (NLR) proteins activate pyroptotic cell death and IL-1 driven inflammation by assembling and activating the inflammasome complex. Closely related sensor proteins NLRP1 and CARD8 undergo unique auto-proteolysis-dependent activation and are implicated in auto-inflammatory diseases; however, their mechanisms of activation are not understood. Here we report the structural basis of how the activating domains (FIIND(UPA)-CARD) of NLRP1 and CARD8 self-oligomerize to assemble distinct inflammasome complexes. Recombinant FIIND(UPA)-CARD of NLRP1 forms a two-layered filament, with an inner core of oligomerized CARD surrounded by an outer ring of FIIND(UPA). Biochemically, self-assembled NLRP1-CARD filaments are sufficient to drive ASC speck formation in cultured human cells-a process that is greatly enhanced by NLRP1-FIIND(UPA) which forms oligomers in vitro. The cryo-EM structures of NLRP1-CARD and CARD8-CARD filaments, solved here at 3.7 A, uncover unique structural features that enable NLRP1 and CARD8 to discriminate between ASC and pro-caspase-1. In summary, our findings provide structural insight into the mechanisms of activation for human NLRP1 and CARD8 and reveal how highly specific signaling can be achieved by heterotypic CARD interactions within the inflammasome complexes.
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Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8.,Gong Q, Robinson K, Xu C, Huynh PT, Chong KHC, Tan EYJ, Zhang J, Boo ZZ, Teo DET, Lay K, Zhang Y, Lim JSY, Goh WI, Wright G, Zhong FL, Reversade B, Wu B Nat Commun. 2021 Jan 8;12(1):188. doi: 10.1038/s41467-020-20319-5. PMID:33420028<ref>PMID:33420028</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6k9f" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Boo, Z Z]]
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[[Category: Boo ZZ]]
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[[Category: Gong, Q]]
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[[Category: Gong Q]]
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[[Category: Wu, B]]
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[[Category: Wu B]]
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[[Category: Xu, C]]
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[[Category: Xu C]]
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[[Category: Zhang, J]]
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[[Category: Zhang J]]
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[[Category: Filament]]
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[[Category: Signaling protein]]
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Current revision

Structure of unknow protein 4

PDB ID 6k9f

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