This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


7d55

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:52, 27 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='7d55' size='340' side='right'caption='[[7d55]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
<StructureSection load='7d55' size='340' side='right'caption='[[7d55]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[7d55]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_phage_phim1ef22 Enterococcus phage phim1ef22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D55 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[7d55]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_phage_phiM1EF22 Enterococcus phage phiM1EF22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D55 FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHIM1EF22_0110 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2218498 Enterococcus phage phiM1EF22])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.397&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d55 OCA], [https://pdbe.org/7d55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d55 RCSB], [https://www.ebi.ac.uk/pdbsum/7d55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d55 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d55 OCA], [https://pdbe.org/7d55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d55 RCSB], [https://www.ebi.ac.uk/pdbsum/7d55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d55 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A2Z6FZW5_9CAUD A0A2Z6FZW5_9CAUD]
-
Lysins are a class of hydrolytic enzymes used by bacteriophages to target and cleave the peptidoglycan of bacterial cell walls during their lytic cycle. The lysins from bacteriophages that infect Gram-positive bacteria are typically monomeric and consist of one or two catalytic domains (CD) and a cell binding domain (CBD). However, multimeric lysins encoded by a single gene have also been reported, among which Lys170 from enterococcal phage F170/08 was one of the first identified. Here, we determined the crystal structure of Lys170 CBD at 1.40 A resolution. The structure reveals that Lys170 CBDs assemble into a tetrameric functional unit and that each monomer folds into a three-stranded beta-sheet core capped on each side by an alpha-helix. In addition, we identified key residues of Lys170 CBD involved in host cell binding. Our work provides a basis for designing highly efficient lysins targeting Enterococcus faecalis.
+
-
 
+
-
Structural and biochemical analyses of the tetrameric cell binding domain of Lys170 from enterococcal phage F170/08.,Xu X, Zhang D, Zhou B, Zhen X, Ouyang S Eur Biophys J. 2021 Feb 20. pii: 10.1007/s00249-021-01511-x. doi:, 10.1007/s00249-021-01511-x. PMID:33609147<ref>PMID:33609147</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 7d55" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Enterococcus phage phim1ef22]]
+
[[Category: Enterococcus phage phiM1EF22]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Zhen, X]]
+
[[Category: Zhen X]]
-
[[Category: Hydrolase]]
+
-
[[Category: Lysin]]
+

Current revision

Crystal structure of lys170 CBD

PDB ID 7d55

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7d55"
Personal tools