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7dh8

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Crystal structure of holo XcZur

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 <StructureSection load='7dh8' size='340' side='right'caption='[[7dh8]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7dh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanc8 Xanc8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DH8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7dh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris_str._8004 Xanthomonas campestris pv. campestris str. 8004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DH8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zur, XC_1430 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=314565 XANC8])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dh8 OCA], [https://pdbe.org/7dh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dh8 RCSB], [https://www.ebi.ac.uk/pdbsum/7dh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dh8 ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/Q4UWS5_XANC8 Q4UWS5_XANC8]
-The zinc uptake regulator (Zur) is a member of the Fur (ferric uptake regulator) family transcriptional regulators that plays important roles in zinc homeostasis and virulence of bacteria. Upon zinc perception, Zur binds to the promoters of zinc responsive genes and controls their transcription. However, the mechanism underlying zinc-mediated Zur activation remains unclear. Here we report a 2.2-A crystal structure of apo Zur from the phytopathogen Xanthomonas campestris pv. campestris (XcZur), which reveals the molecular mechanism that XcZur exists in a closed inactive state before regulatory zinc binding. Subsequently, we present a 1.9-A crystal structure of holo XcZur, which, by contrast, adopts an open state that has enough capacity to bind DNA. Structural comparison and hydrogen deuterium exchange mass spectrometry (HDX-MS) analyses uncover that binding of a zinc atom in the regulatory site, formed by the hinge region, the dimerization domain and the DNA binding domain, drives a closed-to-open conformational change that is essential for XcZur activation. Moreover, key residues responsible for DNA recognition are identified by site-directed mutagenesis. This work provides important insights into zinc-induced XcZur activation and valuable discussions on the mechanism of DNA recognition.
-Structural basis for zinc-induced activation of a zinc uptake transcriptional regulator.,Liu F, Su Z, Chen P, Tian X, Wu L, Tang DJ, Li P, Deng H, Ding P, Fu Q, Tang JL, Ming Z Nucleic Acids Res. 2021 May 28. pii: 6287845. doi: 10.1093/nar/gkab432. PMID:34048589<ref>PMID:34048589</ref>
+==See Also==
+*[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]]
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 7dh8" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Xanc8]]
+[[Category: Xanthomonas campestris pv. campestris str. 8004]]
-[[Category: Chen, P]]
+[[Category: Chen P]]
-[[Category: Deng, H T]]
+[[Category: Deng HT]]
-[[Category: Li, P F]]
+[[Category: Li PF]]
-[[Category: Liu, F M]]
+[[Category: Liu FM]]
-[[Category: Ming, Z H]]
+[[Category: Ming ZH]]
-[[Category: Su, Z H]]
+[[Category: Su ZH]]
-[[Category: Tang, D J]]
+[[Category: Tang DJ]]
-[[Category: Tang, J L]]
+[[Category: Tang JL]]
-[[Category: Tian, X L]]
+[[Category: Tian XL]]
-[[Category: Wu, L J]]
+[[Category: Wu LJ]]
-[[Category: Conformational change]]
-[[Category: Dna binding]]
-[[Category: Metal binding protein]]
-[[Category: Zinc perception]]
-[[Category: Zinc uptake regulator]]

7dh8

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Crystal structure of holo XcZur

Structural highlights

Function

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