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1fjr

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(New page: 200px<br /><applet load="1fjr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fjr, resolution 2.30&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1fjr.jpg|left|200px]]<br /><applet load="1fjr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fjr, resolution 2.30&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE ECTODOMAIN OF METHUSELAH'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE ECTODOMAIN OF METHUSELAH==
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The Drosophila mutant methuselah (mth) was identified from a screen for, single gene mutations that extended average lifespan. Mth mutants have a, 35% increase in average lifespan and increased resistance to several forms, of stress, including heat, starvation, and oxidative damage. The protein, affected by this mutation is related to G protein-coupled receptors of the, secretin receptor family. Mth, like secretin receptor family members, has, a large N-terminal ectodomain, which may constitute the ligand binding, site. Here we report the 2.3-A resolution crystal structure of the Mth, extracellular region, revealing a folding topology in which three, primarily beta-structure-containing domains meet to form a shallow, interdomain groove containing a solvent-exposed tryptophan that may, represent a ligand binding site. The Mth structure is analyzed in relation, to predicted Mth homologs and potential ligand binding features.
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<StructureSection load='1fjr' size='340' side='right'caption='[[1fjr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1fjr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJR FirstGlance]. <br>
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1FJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with NAG, SO4 and PB as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FJR OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjr OCA], [https://pdbe.org/1fjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fjr RCSB], [https://www.ebi.ac.uk/pdbsum/1fjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjr ProSAT]</span></td></tr>
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Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-coupled receptor associated with extended lifespan., West AP Jr, Llamas LL, Snow PM, Benzer S, Bjorkman PJ, Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3744-9. Epub 2001 Mar 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11274391 11274391]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTH_DROME MTH_DROME] Involved in biological aging and stress response. Essential for adult survival. Required in the presynaptic motor neuron to up-regulate neurotransmitter exocytosis at larval glutamatergic neuromuscular junctions (NMJs). Regulates a step associated with docking and clustering of vesicles at release sites. Sun, Acp70A/SP and eys/SPAM are agonists that activate mth.<ref>PMID:9794765</ref> <ref>PMID:12367510</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fjr ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Benzer, S.]]
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[[Category: Benzer S]]
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[[Category: Bjorkman, P.J.]]
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[[Category: Bjorkman PJ]]
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[[Category: Jr., A.P.West.]]
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[[Category: Llamas LL]]
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[[Category: Llamas, L.L.]]
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[[Category: Snow PM]]
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[[Category: Snow, P.M.]]
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[[Category: West Jr AP]]
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[[Category: NAG]]
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[[Category: PB]]
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[[Category: SO4]]
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[[Category: ectodomain]]
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[[Category: g protein-coupled receptor]]
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[[Category: gpcr]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:59:08 2007''
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Current revision

CRYSTAL STRUCTURE OF THE ECTODOMAIN OF METHUSELAH

PDB ID 1fjr

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