This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1fl3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:13, 27 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fl3.jpg|left|200px]]
 
-
{{Structure
+
==CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K==
-
|PDB= 1fl3 |SIZE=350|CAPTION= <scene name='initialview01'>1fl3</scene>, resolution 2.45&Aring;
+
<StructureSection load='1fl3' size='340' side='right'caption='[[1fl3]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SPB:4-(4-STYRYL-PHENYLCARBAMOYL)-BUTYRIC+ACID'>SPB</scene>
+
<table><tr><td colspan='2'>[[1fl3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FL3 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPB:4-(4-STYRYL-PHENYLCARBAMOYL)-BUTYRIC+ACID'>SPB</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fl3 OCA], [https://pdbe.org/1fl3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fl3 RCSB], [https://www.ebi.ac.uk/pdbsum/1fl3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fl3 ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fl3 OCA], [http://www.ebi.ac.uk/pdbsum/1fl3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fl3 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/IGG2B_MOUSE IGG2B_MOUSE]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/1fl3_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fl3 ConSurf].
 +
<div style="clear:both"></div>
-
'''CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K'''
+
==See Also==
-
 
+
*[[Antibody 3D structures|Antibody 3D structures]]
-
 
+
*[[Sandbox 20009|Sandbox 20009]]
-
==Overview==
+
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-
The forte of catalytic antibodies has resided in the control of the ground-state reaction coordinate. A principle and method are now described in which antibodies can direct the outcome of photophysical and photochemical events that take place on excited-state potential energy surfaces. The key component is a chemically reactive optical sensor that provides a direct report of the dynamic interplay between protein and ligand at the active site. To illustrate the concept, we used a trans-stilbene hapten to elicit a panel of monoclonal antibodies that displayed a range of fluorescent spectral behavior when bound to a trans-stilbene substrate. Several antibodies yielded a blue fluorescence indicative of an excited-state complex or "exciplex" between trans-stilbene and the antibody. The antibodies controlled the isomerization coordinate of trans-stilbene and dynamically coupled this manifold with an active-site residue. A step was taken toward the use of antibody-based photochemical sensors for diagnostic and clinical applications.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1FL3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FL3 OCA].
+
-
 
+
-
==Reference==
+
-
Blue-fluorescent antibodies., Simeonov A, Matsushita M, Juban EA, Thompson EH, Hoffman TZ, Beuscher AE 4th, Taylor MJ, Wirsching P, Rettig W, McCusker JK, Stevens RC, Millar DP, Schultz PG, Lerner RA, Janda KD, Science. 2000 Oct 13;290(5490):307-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11030644 11030644]
+
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Protein complex]]
+
[[Category: Beuscher IV AE]]
-
[[Category: IV, A E.Beuscher.]]
+
[[Category: Stevens RC]]
-
[[Category: Stevens, R C.]]
+
-
[[Category: immunoglobulin fold]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:25:00 2008''
+

Current revision

CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K

PDB ID 1fl3

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fl3"
Personal tools