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1flj

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[[Image:1flj.jpg|left|200px]]
 
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==CRYSTAL STRUCTURE OF S-GLUTATHIOLATED CARBONIC ANHYDRASE III==
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The line below this paragraph, containing "STRUCTURE_1flj", creates the "Structure Box" on the page.
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<StructureSection load='1flj' size='340' side='right'caption='[[1flj]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1flj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FLJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1flj| PDB=1flj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1flj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1flj OCA], [https://pdbe.org/1flj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1flj RCSB], [https://www.ebi.ac.uk/pdbsum/1flj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1flj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAH3_RAT CAH3_RAT] Reversible hydration of carbon dioxide. A major participant in the liver response to oxidative stress.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/1flj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1flj ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF S-GLUTATHIOLATED CARBONIC ANHYDRASE III'''
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==See Also==
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*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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S-Glutathiolation of carbonic anhydrase III (CAIII) occurs rapidly in hepatocytes under oxidative stress. The crystal structure of the S-glutathiolated CAIII from rat liver reveals covalent adducts on cysteines 183 and 188. Electrostatic charge and steric contacts at each modification site inversely correlate with the relative rates of reactivity of these cysteines toward glutathione (GSH). Diffuse electron density associated with the GSH adducts suggests a lack of preferred bonding interactions between CAIII and the glutathionyl moieties. Hence, the GSH adducts are available for binding by a protein capable of reducing this mixed disulfide. These properties are consistent with the participation of CAIII in the protection/recovery from the damaging effects of oxidative agents.
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[[Category: Large Structures]]
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==About this Structure==
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1FLJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLJ OCA].
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==Reference==
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Crystal structure of S-glutathiolated carbonic anhydrase III., Mallis RJ, Poland BW, Chatterjee TK, Fisher RA, Darmawan S, Honzatko RB, Thomas JA, FEBS Lett. 2000 Oct 6;482(3):237-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11024467 11024467]
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[[Category: Carbonate dehydratase]]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Chatterjee TK]]
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[[Category: Chatterjee, T K.]]
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[[Category: Darmawan S]]
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[[Category: Darmawan, S.]]
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[[Category: Fisher RA]]
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[[Category: Fisher, R A.]]
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[[Category: Honzatko RB]]
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[[Category: Honzatko, R B.]]
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[[Category: Mallis RJ]]
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[[Category: Mallis, R J.]]
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[[Category: Poland BW]]
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[[Category: Poland, B W.]]
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[[Category: Thomas JA]]
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[[Category: Thomas, J A.]]
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[[Category: Carbonic anhydrase iii]]
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[[Category: Glutathione]]
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[[Category: S-glutathiolated]]
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[[Category: S-glutathionylated]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:28:07 2008''
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Current revision

CRYSTAL STRUCTURE OF S-GLUTATHIOLATED CARBONIC ANHYDRASE III

PDB ID 1flj

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