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1flr

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(New page: 200px<br /><applet load="1flr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1flr, resolution 1.85&Aring;" /> '''4-4-20 FAB FRAGMENT'...)
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[[Image:1flr.jpg|left|200px]]<br /><applet load="1flr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1flr, resolution 1.85&Aring;" />
 
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'''4-4-20 FAB FRAGMENT'''<br />
 
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==Overview==
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==4-4-20 FAB FRAGMENT==
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The crystal complex of fluorescein bound to the high-affinity, anti-fluorescein 4-4-20 Fab (Ka = 10(10) M-1 at 2 degrees C) has been, determined at 1.85 A. Isomorphous crystals of two isoelectric forms (pI =, 7.5 and 7.9) of the anti-fluorescein 4-4-20 Fab, an IgG2A [Gibson et al., (1988) Proteins: Struct. Funct. Genet., 3, 155-160], have been grown. Both, complexes crystallize with one molecule in the asymmetric unit in space, group P1, with a = 42.75 A, b = 43.87 A, c = 58.17 A, alpha = 95.15, degrees, beta = 86.85 degrees and gamma = 98.01 degrees. The final, structure has an R value of 0.188 at 1.85 A resolution. Interactions, between bound fluorescein, the complementarity-determining regions (CDRs), of the Fab and the active-site mutants of the 4-4-20 single-chain Fv will, be discussed. Differences were found between the structure reported here, and the previously reported 2.7 A 4-4-20 Fab structure [Herron et al., (1989) Proteins: Struct. Funct. Genet., 5, 271-280]. Our structure, determination was based on 26,328 unique reflections--four times the, amount of data used in the previous report. Differences in the two, structures could be explained by differences in interpreting the electron, density maps at the various resolutions. The r.m.s. deviations between the, variable and constant domains of the two structures were 0.77 and 1.54 A, respectively. Four regions of the light chain and four regions of the, heavy chain had r.m.s. backbone deviations of &gt; 4 A. The most significant, of these was the conformation of the light chain CDR 1.
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<StructureSection load='1flr' size='340' side='right'caption='[[1flr]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1flr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FLR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLU:2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-BENZOIC+ACID'>FLU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1flr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1flr OCA], [https://pdbe.org/1flr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1flr RCSB], [https://www.ebi.ac.uk/pdbsum/1flr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1flr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCAM_MOUSE GCAM_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/1flr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1flr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with FLU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FLR OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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1.85 A structure of anti-fluorescein 4-4-20 Fab., Whitlow M, Howard AJ, Wood JF, Voss EW Jr, Hardman KD, Protein Eng. 1995 Aug;8(8):749-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8637844 8637844]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Whitlow M]]
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[[Category: Whitlow, M.]]
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[[Category: FLU]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:00:46 2007''
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Current revision

4-4-20 FAB FRAGMENT

PDB ID 1flr

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