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1fm5

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CRYSTAL STRUCTURE OF HUMAN CD69

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Retrieved from "http://52.214.119.220/wiki/index.php/1fm5"

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-[[Image:1fm5.gif|left|200px]]<br />
-<applet load="1fm5" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1fm5, resolution 2.27&Aring;" />
-'''CRYSTAL STRUCTURE OF HUMAN CD69'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF HUMAN CD69==
-CD69 is a widely expressed type II transmembrane glycoprotein related to, the C-type animal lectins that exhibits regulated expression on a variety, of cells of the hematopoietic lineage, including neutrophils, monocytes, T, cells, B cells, natural killer (NK) cells, and platelets. Activation of T, lymphocytes results in the induced expression of CD69 at the cell surface., In addition, cross-linking of CD69 by specific antibodies leads to the, activation of cells bearing this receptor and to the induction of effector, functions. However, the physiological ligand of CD69 is unknown. We report, here the X-ray crystal structure of the extracellular C-type lectin-like, domain (CTLD) of human CD69 at 2.27 A resolution. Recombinant CD69 was, expressed in bacterial inclusion bodies and folded in vitro. The protein, which exists as a disulfide-linked homodimer on the cell surface, crystallizes as a symmetrical dimer, similar to those formed by the, related NK cell receptors Ly49A and CD94. The structure reveals, conservation of the C-type lectin-like fold, including preservation of the, two alpha-helical regions found in Ly49A and mannose-binding protein, (MBP). However, only one of the nine residues coordinated to Ca(2+) in MBP, is conserved in CD69 and no bound Ca(2+) is evident in the crystal, structure. Surprisingly, electron density suggestive of a puckered, six-membered ring was discovered at a site structurally analogous to the, ligand-binding sites of MBP and Ly49A. This sugar-like density may, represent, or mimic, part of the natural ligand recognized by CD69.
+<StructureSection load='1fm5' size='340' side='right'caption='[[1fm5]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1fm5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FM5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fm5 OCA], [https://pdbe.org/1fm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fm5 RCSB], [https://www.ebi.ac.uk/pdbsum/1fm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fm5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CD69_HUMAN CD69_HUMAN] Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/1fm5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fm5 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-FM5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FM5 OCA].
+*[[CD69|CD69]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of human CD69: a C-type lectin-like activation marker of hematopoietic cells., Natarajan K, Sawicki MW, Margulies DH, Mariuzza RA, Biochemistry. 2000 Dec 5;39(48):14779-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11101293 11101293]
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Margulies, D.H.]]
+[[Category: Margulies DH]]
-[[Category: Mariuzza, R.A.]]
+[[Category: Mariuzza RA]]
-[[Category: Natarajan, K.]]
+[[Category: Natarajan K]]
-[[Category: Sawicki, M.W.]]
+[[Category: Sawicki MW]]
-[[Category: c-type lectin]]
-[[Category: c-type lectin-like domain]]
-[[Category: lectin]]
-[[Category: natural killer cell receptor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:55:02 2007''

1fm5

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CRYSTAL STRUCTURE OF HUMAN CD69

Structural highlights

Function

Evolutionary Conservation

See Also

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