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1fn4

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(New page: 200px<br /> <applet load="1fn4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fn4, resolution 2.80&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1fn4.gif|left|200px]]<br />
 
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<applet load="1fn4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fn4, resolution 2.80&Aring;" />
 
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'''CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOLINE RECEPTOR AGAINST MYASTHENOGENIC ANTIBODIES'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOLINE RECEPTOR AGAINST MYASTHENOGENIC ANTIBODIES==
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The crystal structure of the Fab fragment of the rat monoclonal antibody, 198, with protective activity for the main immunogenic region of the human, muscle acetylcholine receptor against the destructive action of myasthenic, antibodies, has been determined and refined to 2.8 A resolution by X-ray, crystallographic methods. The mouse anti-lysozyme Fab D1.3 was used as a, search model in molecular replacement with the AMORE software. The, complementarity determining regions (CDR)-L2, CDR-H1 and CDR-H2 belong to, canonical groups. Loops CDR-L3, CDR-H2 and CDR-H3, which seem to make a, major contribution to binding, were analyzed and residues of potential, importance for antigen-binding are examined. The antigen-binding site was, found to be a long crescent-shaped crevice. The structure should serve as, a model in the rational design of very high affinity humanized mutants of, Fab198, appropriate for therapeutic approaches in the model autoimmune, disease myasthenia gravis.
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<StructureSection load='1fn4' size='340' side='right'caption='[[1fn4]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fn4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FN4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn4 OCA], [https://pdbe.org/1fn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fn4 RCSB], [https://www.ebi.ac.uk/pdbsum/1fn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KACB_RAT KACB_RAT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fn4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fn4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FN4 OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Fab198, an efficient protector of the acetylcholine receptor against myasthenogenic antibodies., Poulas K, Eliopoulos E, Vatzaki E, Navaza J, Kontou M, Oikonomakos N, Acharya KR, Tzartos SJ, Eur J Biochem. 2001 Jul;268(13):3685-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11432734 11432734]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Eliopoulos, E.]]
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[[Category: Eliopoulos E]]
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[[Category: Kontou, M.]]
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[[Category: Kontou M]]
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[[Category: Navaza, J.]]
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[[Category: Navaza J]]
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[[Category: Poulas, K.]]
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[[Category: Poulas K]]
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[[Category: Vatzaki, E.]]
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[[Category: Vatzaki E]]
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[[Category: fab198]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:30:28 2007''
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Current revision

CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOLINE RECEPTOR AGAINST MYASTHENOGENIC ANTIBODIES

PDB ID 1fn4

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