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1fnu

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[[Image:1fnu.gif|left|200px]]<br /><applet load="1fnu" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fnu, resolution 1.94&Aring;" />
 
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'''STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A'''<br />
 
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==Overview==
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==STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A==
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The streptococcal pyrogenic toxins A, B, and C (SPEA, SPEB, and SPEC) are responsible for the fever, rash, and other toxicities associated with scarlet fever and streptococcal toxic shock syndrome. This role, together with the ubiquity of diseases caused by Streptococcus pyogenes, have prompted structural analyses of SPEA by several groups. Papageorgiou et al. (1999) have recently reported the structure of SPEA crystallized in the absence of zinc. Zinc has been shown to be important in the ability of some staphylococcal and streptococcal toxins to stimulate proliferation of CD4+ T-cells. Since cadmium is more electron dense than zinc and typically binds interchangeably, we grew crystals in the presence of 10 mM CdCl2. Crystals have been obtained in three space groups, and the structure in the P2(1)2(1)2(1) crystal form has been refined to 1.9 A resolution. The structural analysis revealed an identical tetramer as well as a novel tetrahedral cluster of cadmium in all three crystal forms on a disulfide loop encompassing residues 87-98. No cadmium was bound at the site homologous to the zinc site in staphylococcal enterotoxins C (SECs) despite the high structural homology between SPEA and SECs. Subsequent soaking of crystals grown in the presence of cadmium in 10 mM ZnCl2 showed that zinc binds in this site (indicating it can discriminate between zinc and cadmium ions) using the three ligands (Asp77, His106, and His110) homologous to the SECs plus a fourth ligand (Glu33).
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<StructureSection load='1fnu' size='340' side='right'caption='[[1fnu]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fnu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_T12 Streptococcus phage T12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FNU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fnu OCA], [https://pdbe.org/1fnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fnu RCSB], [https://www.ebi.ac.uk/pdbsum/1fnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fnu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPEA_BPT12 SPEA_BPT12]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fnu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fnu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FNU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FNU OCA].
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*[[Exotoxin 3D structures|Exotoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of streptococcal pyrogenic exotoxin A reveals a novel metal cluster., Earhart CA, Vath GM, Roggiani M, Schlievert PM, Ohlendorf DH, Protein Sci. 2000 Sep;9(9):1847-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11045630 11045630]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptococcus phage T12]]
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[[Category: Streptococcus pyogenes]]
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[[Category: Earhart CA]]
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[[Category: Earhart, C A.]]
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[[Category: Ohlendorf DH]]
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[[Category: Ohlendorf, D H.]]
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[[Category: Roggiani M]]
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[[Category: Roggiani, M.]]
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[[Category: Schlievert PM]]
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[[Category: Schlievert, P M.]]
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[[Category: Vath GM]]
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[[Category: Vath, G M.]]
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[[Category: CD]]
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[[Category: exotoxin a]]
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[[Category: superantigen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:39 2008''
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Current revision

STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A

PDB ID 1fnu

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