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1fr8

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(New page: 200px<br /><applet load="1fr8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fr8, resolution 2.4&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1fr8.gif|left|200px]]<br /><applet load="1fr8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fr8, resolution 2.4&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE BOVINE BETA 1,4 GALACTOSYLTRANSFERASE (B4GALT1) CATALYTIC DOMAIN COMPLEXED WITH URIDINE DIPHOSPHOGALACTOSE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE BOVINE BETA 1,4 GALACTOSYLTRANSFERASE (B4GALT1) CATALYTIC DOMAIN COMPLEXED WITH URIDINE DIPHOSPHOGALACTOSE==
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beta1,4-galactosyltransferase T1 (beta4Gal-T1, EC 2.4.1.90/38), a Golgi, resident membrane-bound enzyme, transfers galactose from uridine, diphosphogalactose to the terminal beta-N-acetylglucosamine residues, forming the poly-N-acetyllactosamine core structures present in, glycoproteins and glycosphingolipids. In mammals, beta4Gal-T1 binds to, alpha-lactalbumin, a protein that is structurally homologous to lyzozyme, to produce lactose. beta4Gal-T1 is a member of a large family of, homologous beta4galactosyltransferases that use different types of, glycoproteins and glycolipids as substrates. Here we solved and refined, the crystal structures of recombinant bovine beta4Gal-T1 to 2.4 A, resolution in the presence and absence of the substrate uridine, diphosphogalactose. The crystal structure of the bovine substrate-free, beta4Gal-T1 catalytic domain showed a new fold consisting of a single, conical domain with a large open pocket at its base. In the, substrate-bound complex, the pocket encompassed residues interacting with, uridine diphosphogalactose. The structure of the complex contained clear, regions of electron density for the uridine diphosphate portion of the, substrate, where its beta-phosphate group was stabilized by, hydrogen-bonding contacts with conserved residues including the, Asp252ValAsp254 motif. These results help the interpretation of engineered, beta4Gal-T1 point mutations. They suggest a mechanism possibly involved in, galactose transfer and enable identification of the critical amino acids, involved in alpha-lactalbumin interactions.
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<StructureSection load='1fr8' size='340' side='right'caption='[[1fr8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fr8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FR8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDU:GALACTOSE-URIDINE-5-DIPHOSPHATE'>GDU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fr8 OCA], [https://pdbe.org/1fr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fr8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fr8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B4GT1_BOVIN B4GT1_BOVIN] The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/1fr8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fr8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FR8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with GDU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Beta-N-acetylglucosaminylglycopeptide_beta-1,4-galactosyltransferase Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.38 2.4.1.38] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FR8 OCA].
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of the bovine beta4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose., Gastinel LN, Cambillau C, Bourne Y, EMBO J. 1999 Jul 1;18(13):3546-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10393171 10393171]
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[[Category: Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase]]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bourne, Y.]]
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[[Category: Bourne Y]]
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[[Category: Cambillau, C.]]
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[[Category: Cambillau C]]
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[[Category: Gastinel, L.N.]]
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[[Category: Gastinel LN]]
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[[Category: GDU]]
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[[Category: nucleotide binding protein alpha beta alpha fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:10:55 2007''
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Current revision

CRYSTAL STRUCTURE OF THE BOVINE BETA 1,4 GALACTOSYLTRANSFERASE (B4GALT1) CATALYTIC DOMAIN COMPLEXED WITH URIDINE DIPHOSPHOGALACTOSE

PDB ID 1fr8

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