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1frv

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CRYSTAL STRUCTURE OF THE OXIDIZED FORM OF NI-FE HYDROGENASE

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Retrieved from "http://52.214.119.220/wiki/index.php/1frv"

Categories: Large Structures | Megalodesulfovibrio gigas | Fontecilla-Camps JC | Frey M | Volbeda A

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-[[Image:1frv.gif|left|200px]]<br /><applet load="1frv" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1frv, resolution 2.85&Aring;" />
-'''CRYSTAL STRUCTURE OF THE OXIDIZED FORM OF NI-FE HYDROGENASE'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE OXIDIZED FORM OF NI-FE HYDROGENASE==
-The X-ray structure of the heterodimeric Ni-Fe hydrogenase from, Desulfovibrio gigas, the enzyme responsible for the metabolism of, molecular hydrogen, has been solved at 2.85 A resolution. The active site, which appears to contain, besides nickel, a second metal ion, is buried in, the 60K subunit. The 28K subunit, which coordinates one [3Fe-4S] and two, [4Fe-4S] clusters, contains an amino-terminal domain with similarities to, the redox protein flavodoxin. The structure suggests plausible electron, and proton transfer pathways.
+<StructureSection load='1frv' size='340' side='right'caption='[[1frv]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1frv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Megalodesulfovibrio_gigas Megalodesulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FRV FirstGlance]. <br>
-FRV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas] with NI, SF4, F3S and FEL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytochrome-c3_hydrogenase Cytochrome-c3 hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.2.1 1.12.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FRV OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FEL:HYDRATED+FE'>FEL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1frv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1frv OCA], [https://pdbe.org/1frv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1frv RCSB], [https://www.ebi.ac.uk/pdbsum/1frv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1frv ProSAT]</span></td></tr>
-Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas., Volbeda A, Charon MH, Piras C, Hatchikian EC, Frey M, Fontecilla-Camps JC, Nature. 1995 Feb 16;373(6515):580-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7854413 7854413]
+</table>
-[[Category: Cytochrome-c3 hydrogenase]]
+== Function ==
-[[Category: Desulfovibrio gigas]]
+[https://www.uniprot.org/uniprot/PHNS_MEGGA PHNS_MEGGA]
-[[Category: Protein complex]]
+== Evolutionary Conservation ==
-[[Category: Fontecilla-Camps, J.C.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Frey, M.]]
+Check<jmol>
-[[Category: Volbeda, A.]]
+  <jmolCheckbox>
-[[Category: F3S]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/1frv_consurf.spt"</scriptWhenChecked>
-[[Category: FEL]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: NI]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: SF4]]
+  </jmolCheckbox>
-[[Category: ni-fe hydrogenase]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1frv ConSurf].
-[[Category: oxidoreductase]]
+<div style="clear:both"></div>
+__TOC__
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:12:38 2007''
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Megalodesulfovibrio gigas]]
+[[Category: Fontecilla-Camps JC]]
+[[Category: Frey M]]
+[[Category: Volbeda A]]

1frv

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CRYSTAL STRUCTURE OF THE OXIDIZED FORM OF NI-FE HYDROGENASE

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Evolutionary Conservation

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