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1ft6

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(New page: 200px<br /><applet load="1ft6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ft6, resolution 1.8&Aring;" /> '''REDUCED STATE OF CYTO...)
Current revision (11:16, 27 March 2024) (edit) (undo)
 
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[[Image:1ft6.jpg|left|200px]]<br /><applet load="1ft6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ft6, resolution 1.8&Aring;" />
 
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'''REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA'''<br />
 
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==Overview==
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==REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA==
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Cytochrome c554 (cyt c554) is a tetra-heme cytochrome involved in the, oxidation of NH3 by Nitrosomonas europaea. The X-ray crystal structures of, both the oxidized and dithionite-reduced states of cyt c554 in a new, rhombohedral crystal form have been solved by molecular replacement, at, 1.6 A and 1.8 A resolution, respectively. Upon reduction, the conformation, of the polypeptide chain changes between residues 175 and 179, which are, adjacent to hemes III and IV. Cyt c554 displays conserved heme-packing, motifs that are present in other heme-containing proteins. Comparisons to, hydroxylamine oxidoreductase, the electron donor to cyt c554, and, cytochrome c nitrite reductase, an enzyme involved in nitrite, ammonification, reveal substantial structural similarity in the, polypeptide chain surrounding the heme core environment. The structural, determinants of these heme-packing motifs extend to the buried water, molecules that hydrogen bond to the histidine ligands to the heme iron. In, the original structure determination of a tetragonal crystal form, a cis, peptide bond between His129 and Phe130 was identified that appeared to be, stabilized by crystal contacts. In the rhombohedral crystal form used in, the present high-resolution structure determination, this peptide bond, adopts the trans conformation, but with disallowed angles of phi and psi.
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<StructureSection load='1ft6' size='340' side='right'caption='[[1ft6]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ft6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FT6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTN:DITHIONITE'>DTN</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ft6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ft6 OCA], [https://pdbe.org/1ft6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ft6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ft6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ft6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/C554_NITEU C554_NITEU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/1ft6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ft6 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea] with SO3, PO4, HEM and DTN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FT6 OCA].
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea., Iverson TM, Arciero DM, Hooper AB, Rees DC, J Biol Inorg Chem. 2001 Apr;6(4):390-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11372197 11372197]
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[[Category: Large Structures]]
[[Category: Nitrosomonas europaea]]
[[Category: Nitrosomonas europaea]]
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[[Category: Single protein]]
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[[Category: Arciero DM]]
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[[Category: Arciero, D.M.]]
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[[Category: Hooper AB]]
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[[Category: Hooper, A.B.]]
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[[Category: Iverson TM]]
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[[Category: Iverson, T.M.]]
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[[Category: Rees DC]]
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[[Category: Rees, D.C.]]
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[[Category: DTN]]
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[[Category: HEM]]
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[[Category: PO4]]
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[[Category: SO3]]
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[[Category: heme-stacking]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:15:20 2007''
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Current revision

REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA

PDB ID 1ft6

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