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1ftc

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(New page: 200px<br /><applet load="1ftc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ftc, resolution 2.35&Aring;" /> '''Y13C MUTANT OF AZOTO...)
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[[Image:1ftc.jpg|left|200px]]<br /><applet load="1ftc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ftc, resolution 2.35&Aring;" />
 
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'''Y13C MUTANT OF AZOTOBACTER VINELANDII FDI'''<br />
 
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==Overview==
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==Y13C MUTANT OF AZOTOBACTER VINELANDII FDI==
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Ferredoxins that contain [4Fe-4S]2+/+ clusters often obtain three of their, four cysteine ligands from a highly conserved CysXXCysXXCys sequence, motif. Little is known about the in vivo assembly of these clusters and, the role that this sequence motif plays in that process. In this study, we, have used structure as a guide in attempts to direct the formation of a, [4Fe-4S]2+/+ in the [3Fe-4S]+/0 location of native (7Fe) Azotobacter, vinelandii ferredoxin I (AvFdI) by providing the correct three-dimensional, orientation of cysteine ligands without introducing a CysXXCysXXCys motif., Tyr13 of AvFdI occupies the position of the fourth ligating cysteine in, the homologous and structurally characterized 8Fe ferredoxin from, Peptococcus aerogenes and a Y13C variant of AvFdI could be easily modeled, as an 8Fe protein. However, characterization of purified Y13C FdI by, UV-visible spectra, circular dichroism, electron paramagnetic resonance, spectroscopies, and by x-ray crystallography revealed that the protein, failed to use the introduced cysteine as a ligand and retained its, [3Fe-4S]+/0 cluster. Further, electrochemical characterization showed that, the redox potential and pH behavior of the cluster were unaffected by the, substitution of Tyr by Cys. Although Y13C FdI is functional in vivo it, does differ significantly from native FdI in that it is extremely unstable, in the reduced state possibly due to increased solvent exposure of the, [3Fe-4S]0 cluster. Surprisingly, the x-ray structure showed that the, introduced cysteine was modified to become a persulfide. This modification, may have occurred in vivo via the action of NifS, which is known to be, expressed under the growth conditions used. It is interesting to note that, neither of the two free cysteines present in FdI was modified. Thus, if, NifS is involved in modifying the introduced cysteine there must be, specificity to the reaction.
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<StructureSection load='1ftc' size='340' side='right'caption='[[1ftc]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ftc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FTC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ftc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ftc OCA], [https://pdbe.org/1ftc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ftc RCSB], [https://www.ebi.ac.uk/pdbsum/1ftc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ftc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FER1_AZOVI FER1_AZOVI] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin could play a role in regulating gene expression by interacting directly with DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/1ftc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ftc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FTC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with SF4 and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FTC OCA].
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Y13C Azotobacter vinelandii ferredoxin I. A designed [Fe-S] ligand motif contains a cysteine persulfide., Kemper MA, Stout CD, Lloyd SJ, Prasad GS, Fawcett SE, Armstrong FA, Shen B, Burgess BK, J Biol Chem. 1997 Jun 20;272(25):15620-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9188450 9188450]
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[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Armstrong, F.A.]]
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[[Category: Armstrong FA]]
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[[Category: Burgess, B.K.]]
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[[Category: Burgess BK]]
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[[Category: Fawcett, S.]]
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[[Category: Fawcett S]]
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[[Category: Kemper, M.A.]]
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[[Category: Kemper MA]]
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[[Category: Lloyd, S.J.]]
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[[Category: Lloyd SJ]]
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[[Category: Prasad, G.S.]]
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[[Category: Prasad GS]]
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[[Category: Stout, C.D.]]
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[[Category: Stout CD]]
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[[Category: F3S]]
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[[Category: SF4]]
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[[Category: electron transport]]
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[[Category: iron-sulfur]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:15:31 2007''
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Current revision

Y13C MUTANT OF AZOTOBACTER VINELANDII FDI

PDB ID 1ftc

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