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1g66

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(New page: 200px<br /><applet load="1g66" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g66, resolution 0.90&Aring;" /> '''ACETYLXYLAN ESTERASE...)
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[[Image:1g66.jpg|left|200px]]<br /><applet load="1g66" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g66, resolution 0.90&Aring;" />
 
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'''ACETYLXYLAN ESTERASE AT 0.90 ANGSTROM RESOLUTION'''<br />
 
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==Overview==
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==ACETYLXYLAN ESTERASE AT 0.90 ANGSTROM RESOLUTION==
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Acetylxylan esterase (AXEII; 207 amino acids) from Penicillium, purpurogenum has substrate specificities toward acetate esters of, d-xylopyranose residues in xylan and belongs to a new class of alpha/beta, hydrolases. The crystal structure of AXEII has been determined by single, isomorphous replacement and anomalous scattering, and refined at 0.90- and, 1.10-A resolutions with data collected at 85 K and 295 K, respectively., The tertiary structure consists of a doubly wound alpha/beta sandwich, having a central six-stranded parallel beta-sheet flanked by two parallel, alpha-helices on each side. The catalytic residues Ser(90), His(187), and, Asp(175) are located at the C-terminal end of the sheet, an exposed region, of the molecule. The serine and histidine side chains in the 295 K, structure show the frequently observed conformations in which Ser(90) is, trans and the hydroxyl group is in the plane of the imidazole ring of, His(187). However, the structure at 85 K displays an additional, conformation in which Ser(90) side-chain hydroxyl is away from the plane, of the imidazole ring of His(187). The His(187) side chain forms a, hydrogen bond with a sulfate ion and adopts an altered conformation. The, only other known hydrolase that has a similar tertiary structure is, Fusarium solani cutinase. The exposed nature of the catalytic triad, suggests that AXEII is a pure esterase, i.e. an alpha/beta hydrolase with, specificity for nonlipidic polar substrates.
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<StructureSection load='1g66' size='340' side='right'caption='[[1g66]], [[Resolution|resolution]] 0.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g66]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_purpureogenus Talaromyces purpureogenus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G66 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g66 OCA], [https://pdbe.org/1g66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g66 RCSB], [https://www.ebi.ac.uk/pdbsum/1g66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g66 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AXE2_TALPU AXE2_TALPU] Degrades acetylated xylans by cleaving acetyl side groups from the hetero-xylan backbone.<ref>PMID:8756392</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g66_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g66 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G66 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G66 OCA].
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*[[Acetylxylan esterase 3D structures|Acetylxylan esterase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Multiple conformations of catalytic serine and histidine in acetylxylan esterase at 0.90 A., Ghosh D, Sawicki M, Lala P, Erman M, Pangborn W, Eyzaguirre J, Gutierrez R, Jornvall H, Thiel DJ, J Biol Chem. 2001 Apr 6;276(14):11159-66. Epub 2000 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11134051 11134051]
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__TOC__
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[[Category: Acetylesterase]]
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</StructureSection>
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[[Category: Penicillium purpurogenum]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Talaromyces purpureogenus]]
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[[Category: Erman, M.]]
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[[Category: Erman M]]
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[[Category: Eyzaguirre, J.]]
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[[Category: Eyzaguirre J]]
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[[Category: Ghosh, D.]]
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[[Category: Ghosh D]]
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[[Category: Gutierrez, R.]]
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[[Category: Gutierrez R]]
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[[Category: Jornvall, H.]]
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[[Category: Jornvall H]]
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[[Category: Lala, P.]]
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[[Category: Lala P]]
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[[Category: Pangborn, W.]]
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[[Category: Pangborn W]]
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[[Category: Sawicki, M.]]
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[[Category: Sawicki M]]
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[[Category: Thiel, D.J.]]
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[[Category: Thiel DJ]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: acetyl xylopyranose]]
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[[Category: serine hydrolase]]
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[[Category: ultra-high resolution]]
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[[Category: xylan]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:44:41 2007''
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Current revision

ACETYLXYLAN ESTERASE AT 0.90 ANGSTROM RESOLUTION

PDB ID 1g66

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