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1g7y

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[[Image:1g7y.jpg|left|200px]]
 
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{{Structure
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==THE CRYSTAL STRUCTURE OF THE 58KD VEGETATIVE LECTIN FROM THE TROPICAL LEGUME DOLICHOS BIFLORUS==
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|PDB= 1g7y |SIZE=350|CAPTION= <scene name='initialview01'>1g7y</scene>, resolution 2.50&Aring;
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<StructureSection load='1g7y' size='340' side='right'caption='[[1g7y]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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<table><tr><td colspan='2'>[[1g7y]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_unguiculata_subsp._cylindrica Vigna unguiculata subsp. cylindrica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G7Y FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g7y OCA], [https://pdbe.org/1g7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g7y RCSB], [https://www.ebi.ac.uk/pdbsum/1g7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g7y ProSAT]</span></td></tr>
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</table>
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'''THE CRYSTAL STRUCTURE OF THE 58KD VEGETATIVE LECTIN FROM THE TROPICAL LEGUME DOLICHOS BIFLORUS'''
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== Function ==
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[https://www.uniprot.org/uniprot/LEC5_VIGUC LEC5_VIGUC] Metalloglycoprotein, containing Ca, Mg, Mn, and Zn and the carbohydrates galactose, glucosamine, mannose, and fucose. It agglutinates erythrocytes of blood group A1.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The legume lectins are widely used as a model system for studying protein-carbohydrate and protein-protein interactions. They exhibit a fascinating quaternary structure variation, which becomes important when they interact with multivalent glycoconjugates, for instance those on cell surfaces. Recently, it has become clear that certain lectins form weakly associated oligomers. This phenomenon may play a role in the regulation of receptor crosslinking and subsequent signal transduction. The crystal structure of DB58, a dimeric lectin from the legume Dolichos biflorus reveals a separate dimer of a previously unobserved type, in addition to a tetramer consisting of two such dimers. This tetramer resembles that formed by DBL, the seed lectin from the same plant. A single amino acid substitution in DB58 affects the conformation and flexibility of a loop in the canonical dimer interface. This disrupts the formation of a stable DBL-like tetramer in solution, but does not prohibit its formation in suitable conditions, which greatly increases the possibilities for the cross-linking of multivalent ligands. The non-canonical DB58 dimer has a buried symmetrical alpha helix, which can be present in the crystal in either of two antiparallel orientations. Two existing structures and datasets for lectins with similar quaternary structures were reconsidered. A central alpha helix could be observed in the soybean lectin, but not in the leucoagglutinating lectin from Phaseolus vulgaris. The relative position and orientation of the carbohydrate-binding sites in the DB58 dimer may affect its ability to crosslink mulitivalent ligands, compared to the other legume lectin dimers.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/1g7y_consurf.spt"</scriptWhenChecked>
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1G7Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vigna_unguiculata_subsp._cylindrica Vigna unguiculata subsp. cylindrica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7Y OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus., Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T, J Mol Biol. 2001 May 25;309(1):193-201. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11491289 11491289]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g7y ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vigna unguiculata subsp. cylindrica]]
[[Category: Vigna unguiculata subsp. cylindrica]]
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[[Category: Buts, L.]]
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[[Category: Buts L]]
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[[Category: Dao-Thi, M H.]]
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[[Category: Dao-Thi M-H]]
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[[Category: Etzler, M E.]]
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[[Category: Etzler ME]]
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[[Category: Hamelryck, T W.]]
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[[Category: Hamelryck TW]]
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[[Category: Loris, R.]]
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[[Category: Loris R]]
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[[Category: Wyns, L.]]
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[[Category: Wyns L]]
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[[Category: CA]]
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[[Category: MN]]
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[[Category: jelly roll fold]]
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[[Category: lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:27 2008''
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Current revision

THE CRYSTAL STRUCTURE OF THE 58KD VEGETATIVE LECTIN FROM THE TROPICAL LEGUME DOLICHOS BIFLORUS

PDB ID 1g7y

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