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1gk0
From Proteopedia
(Difference between revisions)
(New page: 200px<br /> <applet load="1gk0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gk0, resolution 2.50Å" /> '''STRUCTURE-BASED PRE...) |
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| - | [[Image:1gk0.gif|left|200px]]<br /> | ||
| - | <applet load="1gk0" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1gk0, resolution 2.50Å" /> | ||
| - | '''STRUCTURE-BASED PREDICTION OF MODIFICATIONS IN GLUTARYLAMIDASE TO ALLOW SINGLE-STEP ENZYMATIC PRODUCTION OF 7-AMINOCEPHALOSPORANIC ACID FROM CEPHALOSPORIN C'''<br /> | ||
| - | == | + | ==Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C== |
| - | + | <StructureSection load='1gk0' size='340' side='right'caption='[[1gk0]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1gk0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GK0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gk0 OCA], [https://pdbe.org/1gk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gk0 RCSB], [https://www.ebi.ac.uk/pdbsum/1gk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gk0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G7AC_PSEU7 G7AC_PSEU7] Catalyzes the deacylation of 7 beta-(4-carboxybutanamido)cephalosporanic acid (glutaryl-7-aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA).<ref>PMID:2993240</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gk0_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gk0 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| - | == | + | ==See Also== |
| - | + | *[[Cephalosporin acylase 3D structures|Cephalosporin acylase 3D structures]] | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Pseudomonas sp | + | [[Category: Large Structures]] |
| - | [[Category: Aretz | + | [[Category: Pseudomonas sp]] |
| - | [[Category: Fritz-Wolf | + | [[Category: Aretz W]] |
| - | [[Category: Kabsch | + | [[Category: Fritz-Wolf K]] |
| - | [[Category: Koller | + | [[Category: Kabsch W]] |
| - | [[Category: Lange | + | [[Category: Koller KP]] |
| - | [[Category: Liesum | + | [[Category: Lange G]] |
| - | [[Category: Sauber | + | [[Category: Liesum A]] |
| - | [[Category: Schreuder | + | [[Category: Sauber K]] |
| - | + | [[Category: Schreuder H]] | |
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Current revision
Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C
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Categories: Large Structures | Pseudomonas sp | Aretz W | Fritz-Wolf K | Kabsch W | Koller KP | Lange G | Liesum A | Sauber K | Schreuder H
