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1gmw

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(New page: 200px<br /> <applet load="1gmw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gmw, resolution 1.5&Aring;" /> '''STRUCTURE OF UREE'''...)
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[[Image:1gmw.gif|left|200px]]<br />
 
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<applet load="1gmw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gmw, resolution 1.5&Aring;" />
 
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'''STRUCTURE OF UREE'''<br />
 
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==Overview==
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==Structure of UreE==
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UreE is proposed to be a metallochaperone that delivers nickel ions to, urease during activation of this bacterial virulence factor. Wild-type, Klebsiella aerogenes UreE binds approximately six nickel ions per, homodimer, whereas H144*UreE (a functional C-terminal truncated variant), was previously reported to bind two. We determined the structure of, H144*UreE by multi-wavelength anomalous diffraction and refined it to 1.5, A resolution. The present structure reveals an Hsp40-like peptide-binding, domain, an Atx1-like metal-binding domain, and a flexible C terminus., Three metal-binding sites per dimer, defined by structural analysis of, Cu-H144*UreE, are on the opposite face of the Atx1-like domain than, observed in the copper metallochaperone. One metal bridges the two, subunits via ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11591723 (full description)]]
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<StructureSection load='1gmw' size='340' side='right'caption='[[1gmw]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gmw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GMW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gmw OCA], [https://pdbe.org/1gmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gmw RCSB], [https://www.ebi.ac.uk/pdbsum/1gmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gmw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UREE_KLEAE UREE_KLEAE] Involved in urease metallocenter assembly. Binds about 6 nickel ions per homodimer. Probably functions as a nickel donor during metallocenter assembly. Its function can be bypassed in vitro in the presence of high nickel concentrations.[HAMAP-Rule:MF_00822]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/1gmw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gmw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GMW is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GMW OCA]].
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*[[Urease accessory protein 3D structures|Urease accessory protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation., Song HK, Mulrooney SB, Huber R, Hausinger RP, J Biol Chem. 2001 Dec 28;276(52):49359-64. Epub 2001 Oct 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11591723 11591723]
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[[Category: Klebsiella aerogenes]]
[[Category: Klebsiella aerogenes]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Hausinger, R.]]
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[[Category: Hausinger R]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Mulrooney, S.B.]]
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[[Category: Mulrooney SB]]
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[[Category: Song, H.K.]]
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[[Category: Song HK]]
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[[Category: CU]]
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[[Category: metallochaperone]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:43:06 2007''
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Current revision

Structure of UreE

PDB ID 1gmw

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