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1grn

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(New page: 200px<br /> <applet load="1grn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1grn, resolution 2.1&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1grn.gif|left|200px]]<br />
 
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<applet load="1grn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1grn, resolution 2.1&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE CDC42/CDC42GAP/ALF3 COMPLEX.'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE CDC42/CDC42GAP/ALF3 COMPLEX.==
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The Rho-related small GTP-binding protein Cdc42 has a low intrinsic GTPase, activity that is significantly enhanced by its specific GTPase-activating, protein, Cdc42GAP. In this report, we present the tertiary structure for, the aluminum fluoride-promoted complex between Cdc42 and a catalytically, active domain of Cdc42GAP as well as the complex between Cdc42 and the, catalytically compromised Cdc42GAP(R305A) mutant. These structures, which, mimic the transition state for the GTP hydrolytic reaction, show the, presence of an AIF3 molecule, as was seen for the corresponding, Ras-p120RasGAP complex, but in contrast to what has been reported for the, Rho-Cdc42GAP complex or for heterotrimeric G protein alpha subunits, where, AIF4- was observed. The Cdc42GAP stabilizes both the switch I and switch, II domains of Cdc42 and contributes a highly conserved arginine (Arg 305), to the active site. Comparison of the structures for the wild type and, mutant Cdc42GAP complexes provides important insights into the, GAP-catalyzed GTP hydrolytic reaction.
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<StructureSection load='1grn' size='340' side='right'caption='[[1grn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1grn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GRN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1grn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1grn OCA], [https://pdbe.org/1grn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1grn RCSB], [https://www.ebi.ac.uk/pdbsum/1grn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1grn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/1grn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1grn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GRN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, GDP and AF3 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GRN OCA].
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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*[[Rho GTPase activating protein 3D structures|Rho GTPase activating protein 3D structures]]
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==Reference==
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== References ==
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Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP., Nassar N, Hoffman GR, Manor D, Clardy JC, Cerione RA, Nat Struct Biol. 1998 Dec;5(12):1047-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9846874 9846874]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Cerione, R.A.]]
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[[Category: Cerione RA]]
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[[Category: Clardy, J.C.]]
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[[Category: Clardy JC]]
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[[Category: Hoffman, G.R.]]
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[[Category: Hoffman GR]]
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[[Category: Nassar, N.]]
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[[Category: Nassar N]]
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[[Category: AF3]]
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[[Category: GDP]]
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[[Category: MG]]
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[[Category: alf3]]
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[[Category: cdc42]]
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[[Category: g-protein]]
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[[Category: gap]]
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[[Category: transition-state]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:08:27 2007''
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Current revision

CRYSTAL STRUCTURE OF THE CDC42/CDC42GAP/ALF3 COMPLEX.

PDB ID 1grn

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