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1gz1

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(New page: 200px<br /> <applet load="1gz1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gz1, resolution 1.90&Aring;" /> '''MUTANT D416A OF THE...)
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[[Image:1gz1.gif|left|200px]]<br />
 
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<applet load="1gz1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gz1, resolution 1.90&Aring;" />
 
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'''MUTANT D416A OF THE CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS IN COMPLEX WITH METHYL-CELLOBIOSYL-4-DEOXY-4-THIO-BETA-D-CELLOBIOSIDE'''<br />
 
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==Overview==
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==Mutant D416A of the CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS in complex with methyl-cellobiosyl-4-deoxy-4-thio-beta-D-cellobioside==
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The enzymatic degradation of cellulose continues to be one of the most, important enzyme-catalysed reactions. Glycoside hydrolases from family, GH-6 hydrolyse cellulose with inversion of the configuration of the, anomeric carbon. Whilst the catalytic proton donor has been clearly, identified (Asp226 in Humicola insolens Cel6A), the identification and, even the existence of a potential Bronsted base remains unclear. Equally, controversial is the role of surface-loop flexibility. Here, the structure, of the D416A mutant of the H. insolens cellobiohydrolase Cel6A in complex, with a non-hydrolysable thiooligosaccharide methyl, cellobiosyl-4-thio-beta-cellobioside at 1.9 A resolution is presented., Substrate distortion in the -1 subsite, to a (2)S(0) skew-boat, conformation, is observed, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12454501 (full description)]]
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<StructureSection load='1gz1' size='340' side='right'caption='[[1gz1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gz1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZ1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MGL:O1-METHYL-GLUCOSE'>MGL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz1 OCA], [https://pdbe.org/1gz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gz1 RCSB], [https://www.ebi.ac.uk/pdbsum/1gz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gz1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUX6_HUMIN GUX6_HUMIN] Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.<ref>PMID:9882628</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gz1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gz1 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GZ1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]] with NAG and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZ1 OCA]].
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*[[Cellobiohydrolase 3D structures|Cellobiohydrolase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9 A., Varrot A, Frandsen TP, Driguez H, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2201-4. Epub 2002, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454501 12454501]
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__TOC__
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</StructureSection>
[[Category: Humicola insolens]]
[[Category: Humicola insolens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Davies, G.J.]]
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[[Category: Davies GJ]]
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[[Category: Driguez, H.]]
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[[Category: Driguez H]]
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[[Category: Frandsen, H.]]
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[[Category: Frandsen T]]
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[[Category: Varrot, A.]]
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[[Category: Varrot A]]
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[[Category: NA]]
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[[Category: NAG]]
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[[Category: cellobiohydrolase]]
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[[Category: cellulase]]
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[[Category: cellulose degradation]]
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[[Category: glycoside hydrolase family 6]]
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[[Category: hydrolase]]
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[[Category: thiooligosaccharide]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 17:01:26 2007''
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Current revision

Mutant D416A of the CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS in complex with methyl-cellobiosyl-4-deoxy-4-thio-beta-D-cellobioside

PDB ID 1gz1

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