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1gz5

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[[Image:1gz5.gif|left|200px]]<br />
 
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<applet load="1gz5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gz5, resolution 2.43&Aring;" />
 
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'''TREHALOSE-6-PHOSPHATE SYNTHASE. OTSA'''<br />
 
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==Overview==
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==Trehalose-6-phosphate synthase. OtsA==
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Trehalose is a nonreducing disaccharide that plays a major role in many, organisms, most notably in survival and stress responses. In Mycobacterium, tuberculosis, it plays a central role as the carbohydrate core of numerous, immunogenic glycolipids including "cord factor" (trehalose, 6,6'-dimycolate). The classical pathway for trehalose synthesis involves, the condensation of UDP-glucose and glucose-6-phosphate to afford, trehalose-6-phosphate, catalyzed by the retaining glycosyltransferase, OtsA. The configurations of two anomeric positions are set simultaneously, resulting in the formation of a double glycoside. The three-dimensional, structure of the Escherichia coli OtsA, in complex with both UDP and, glucose-6-phosphate, reveals the active site at the interface of two, beta/alpha/beta domains. The overall structure and the intimate details of, the catalytic machinery reveal a striking similarity to glycogen, phosphorylase, indicating a strong evolutionary link and suggesting a, common catalytic mechanism.
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<StructureSection load='1gz5' size='340' side='right'caption='[[1gz5]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gz5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZ5 FirstGlance]. <br>
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1GZ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with G6P, UDP and IMD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] Structure known Active Site: UDA. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZ5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz5 OCA], [https://pdbe.org/1gz5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gz5 RCSB], [https://www.ebi.ac.uk/pdbsum/1gz5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gz5 ProSAT]</span></td></tr>
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Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA., Gibson RP, Turkenburg JP, Charnock SJ, Lloyd R, Davies GJ, Chem Biol. 2002 Dec;9(12):1337-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12498887 12498887]
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</table>
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[[Category: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)]]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/OTSA_ECOLI OTSA_ECOLI] Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor. Essential for viability of the cells at low temperatures and at elevated osmotic strength.<ref>PMID:1310094</ref> <ref>PMID:3131312</ref> <ref>PMID:12105274</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Davies, G.J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Gibson, R.P.]]
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Check<jmol>
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[[Category: Turkenburg, J.P.]]
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<jmolCheckbox>
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[[Category: G6P]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gz5_consurf.spt"</scriptWhenChecked>
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[[Category: IMD]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: UDP]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: glucose-6-phosphate]]
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</jmolCheckbox>
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[[Category: glycosyltransferase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gz5 ConSurf].
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[[Category: rossmann-fold]]
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<div style="clear:both"></div>
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[[Category: synthase]]
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== References ==
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[[Category: trehalose]]
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<references/>
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[[Category: trehalose-6-phosphate]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:23:18 2007''
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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[[Category: Large Structures]]
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[[Category: Davies GJ]]
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[[Category: Gibson RP]]
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[[Category: Turkenburg JP]]

Current revision

Trehalose-6-phosphate synthase. OtsA

PDB ID 1gz5

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